Skip to main content
Addgene
Showing: 1 - 20 of 44 results

  2. pG1AK-PheB

    Plasmid
    #71738
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Insert
    PheB
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pRplsWT
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71738-plasmid-map-sequence-id-135061

    Enlarge

  3. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87700-plasmid-map-sequence-id-174647

    Enlarge

  4. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87703-plasmid-map-sequence-id-176992

    Enlarge

  5. pGLU_81

    Plasmid
    #225782
    Purpose
    RS421 library variant 13401
    Depositor
    Nili Ostrov
    Insert
    13401_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225782-plasmid-map-sequence-id-446979

    Enlarge

  6. pGLU_99

    Plasmid
    #225812
    Purpose
    RS421 library variant 13401
    Depositor
    Nili Ostrov
    Insert
    13401_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225812-plasmid-map-sequence-id-446977

    Enlarge

  7. pGLU_117

    Plasmid
    #225797
    Purpose
    RS421 library variant 13401
    Depositor
    Nili Ostrov
    Insert
    13401_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225797-plasmid-map-sequence-id-446915

    Enlarge

  9. pG1K

    Plasmid
    #71741
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71741-plasmid-map-sequence-id-130144

    Enlarge

  11. pG1C

    Plasmid
    #71740
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71740-plasmid-map-sequence-id-130141

    Enlarge

  12. pG2K

    Plasmid
    #71742
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71742-plasmid-map-sequence-id-130147

    Enlarge

  13. pG1AK

    Plasmid
    #71736
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71736-plasmid-map-sequence-id-130104

    Enlarge

  14. pG1AK-sfGFP

    Plasmid
    #71739
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Insert
    sfGFP
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pRplsWT
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71739-plasmid-map-sequence-id-130138

    Enlarge

  15. pG1AK-mCherry

    Plasmid
    #71737
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Insert
    mCherry
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pRplsWT
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71737-plasmid-map-sequence-id-130132

    Enlarge

  18. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Article
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Use
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

    Enlarge

  20. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

    Enlarge

Showing: 1 - 20 of 44 results