Addgene

Showing: 1 - 20 of 1288 results

Results per page:

Sort results by:

Agarabi CHO Heavy Chain in pJ201

Plasmid

#111567

Purpose

Cloning vector containing CDS for the modified human heavy chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

Depositor

Cyrus Agarabi

Article

Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

Insert

Human heavy chain

Use

Synthetic Biology; Cloning vector

Tags

Expression

Mutation

Promoter

None

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Agarabi CHO Light Chain in pJ201

Plasmid

#111566

Purpose

Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

Depositor

Cyrus Agarabi

Article

Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

Insert

Murine light chain

Use

Synthetic Biology; Cloning vector

Tags

Expression

Mutation

Promoter

None

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Arabidopsis thaliana Atm3 pET21a+

Plasmid

#172321

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Arabidopsis thaliana Atm3 d80 pET21a+

Plasmid

#173045

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

with N-terminal 80 amino acids deletion

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
gCH29 (crCD81-1)

Plasmid

#217344

Purpose

crRNA targeting CD81

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crCD81-1 (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pG0A0-1-1-PBAD-dCas9

Plasmid

#208815

Purpose

Expresses arabinose-inducible dCas9, codon-optimized for Salmonella

Depositor

Marc Erhardt

Article

Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

Insert

Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Arabinsoe-inducible promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

Plasmid

#217340

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L0I89-pMUTE_GG0-1

Plasmid

#219708

Purpose

Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.

Depositor

Jennifer Nemhauser

Insert

GG0-pMUTE-GG1

Use

Synthetic Biology

Tags

Expression

Mutation

WT promoter with added golden gate linkers

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L0I87-SPCHPromoter_GG0-1

Plasmid

#219707

Purpose

Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.

Depositor

Jennifer Nemhauser

Insert

GG0-pSPCH-GG1

Use

Synthetic Biology

Tags

Expression

Mutation

WT promoter with added golden gate linkers

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM-PAT-ProCDKA;1::CDKA;1:YFP

Plasmid

#80685

Purpose

binary Expression vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Nowack et al Nature. 2007 May 17;447(7142):312-5. Epu

Insert

CDKA;1 (CDC2 Mustard Weed)

Use

Tags

YFP

Expression

Plant

Mutation

Promoter

ProCDKA;1

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mTALEAD nad7-1

Plasmid

#210831

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in nad7 in mitochondria

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EFF-1::V5

Plasmid

#132961

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

EFF-1::V5

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Fragment 1 Null

Plasmid

#198729

Purpose

Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

gRNA expression cassette in pENTR4

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDV12 [psng-1::SNG-1::CRY2olig(535)]

Plasmid

#197596

Purpose

Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)

Use

Tags

Expression

Worm

Mutation

E490G

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-1 (GB1204)

Plasmid

#75405

Purpose

Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

AtU6-1 promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217341

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR207-SD3(1-50)

Plasmid

#44587

Purpose

Gateway entry vector for N-terminal fragment (1-50) of SD3 protein

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

SD3(1-50)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mTALEAD ATP6-2-1

Plasmid

#210829

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in ATP6-2 in mitochondria

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mTALEAD 16S rRNA-1

Plasmid

#210833

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-1-AtUbi10-6

Plasmid

#176871

Purpose

MoClo Level 0 plasmid, contains AtUbi1 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge