1. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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1. pGBW-m4046911

   Plasmid

   #145695

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. AK9 gRNA (BRDN0001146911)

   Plasmid

   #77689

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human AK9

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   AK9 (AKD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

   Plasmid

   #154473

   Purpose

   Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   SFFV

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-lambdaN-HA-DmCup-del580-600_M

   Plasmid

   #146911

   Purpose

   Insect Expression of DmCup-del580-600

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

   Insert

   DmCup-del580-600 (cup Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-UBF

   Plasmid

   #17656

   Depositor

   Tom Misteli

   Article

   Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

   Insert

   UBF (UBTF Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-RPA194

   Plasmid

   #17660

   Depositor

   Tom Misteli

   Article

   Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

   Insert

   RPA194 (Polr1a Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

7. GFP-RPA43

   Plasmid

   #17659

   Depositor

   Tom Misteli

   Article

   Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

   Insert

   RPA43 (Twistnb Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

8. GFP-RPA40

   Plasmid

   #17658

   Depositor

   Tom Misteli

   Article

   Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

   Insert

   RPA40 (POLR1C Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

9. GFP-RPA16

   Plasmid

   #17657

   Depositor

   Tom Misteli

   Article

   Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

   Insert

   RPA16 (POLR1D Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

10. GFP-RRN3/TIF1A

    Plasmid

    #17661

    Depositor

    Tom Misteli

    Article

    Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

    Insert

    RRN3/TIF1A (RRN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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