1. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. hCas9-VPR

   Plasmid

   #68497

   Purpose

   nuclease competent SP-Cas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   SP-Cas9-VPR
   

   Use

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCas9-CtIP

   Plasmid

   #109403

   Purpose

   Expresses Cas9 fusion to human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   CtIP (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   full length CtIP

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas9-HE-Geminin

   Plasmid

   #109402

   Purpose

   Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   HE-Geminin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas9-HE

   Plasmid

   #109400

   Purpose

   Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   HDR-Enhancer domain of human CtIP protein (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas9-Geminin

   Plasmid

   #109401

   Purpose

   Expresses Cas9 fusion to Geminin degron domain

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   Geminin (GMNN Human)
   

   Use

   Tags

   Expression

   Mutation

   Geminin degron fragment

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF1349

   Plasmid

   #141815

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POGZ (POGZ Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RCAS(A) cWnt5b (CT#851)

   Plasmid

   #13975

   Depositor

   Cliff Tabin

   Article

   Theodosiou et al Dev Biol. 2003 Jul 15. 259(2):258-71.

   Insert

   Wnt5b (WNT5B Chicken)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pHCKan-INSyibp-Nef

   Plasmid

   #134593

   Purpose

   Low Phosphate inducible Nef

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   HIV Nef protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHCKan-yibDp-GFPuv

   Plasmid

   #127078

   Purpose

   Low phosphate inducible yibD gene promoter expressing GFPuv reporter

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCKan-yibDp-GST

    Plasmid

    #134592

    Purpose

    Low Phosphate inducible GST

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Glutathione S-transferase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHCKan-yibDp-mCherry

    Plasmid

    #127058

    Purpose

    Low phosphate inducible mCherry with N-terminal linker and His tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDF-yibDp-matB

    Plasmid

    #134597

    Purpose

    Low Phosphate inducible matB (malonyl-CoA synthetase)

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Malonyl CoA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTKhc-yibDp-GFP-cp6

    Plasmid

    #134938

    Purpose

    Low phosphate inducible circular permutation of GFP

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPcp6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHCKan-yibDp-ald*-alaE

    Plasmid

    #134939

    Purpose

    Low phosphate inducible alanine dehydrogenase and alanine exporter

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    alanine dehydrogenase and L-alanine exporter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSMART-GFPuv

    Plasmid

    #65822

    Purpose

    GFPuv Expression, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    gfpuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli insulated waaH gene promoter, low phosp…

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHCKan-yibDp-mdlC

    Plasmid

    #134590

    Purpose

    Low Phosphate inducible mdlC benzylformate decarboxylase

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mdlC (benzylformate decarboxylase) C -terminal his tag
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHCKan-yibDp-cimA3.7

    Plasmid

    #134595

    Purpose

    Low Phosphate inducible mutant citramalate synthase

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    cimA3.7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I47V, E114V, H126Q, T204A, L238S

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHCKan-yibDp-SBS-mCherry

    Plasmid

    #134598

    Purpose

    Low Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    SBS-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHCKan-T7-CBD-hGLY

    Plasmid

    #134940

    Purpose

    T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Use

    Tags

    chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge