Skip to main content
Addgene
Showing: 1 - 20 of 365 results

  6. pET29a-YS14

    Plasmid
    #66890
    Purpose
    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)
    Depositor
    Jung-Hyun Min
    Article
    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.
    Inserts
    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    Use
    Tags
    H2A, H2B, H3, H4, His6 tag, and Thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    66890-plasmid-map-sequence-id-305064

    Enlarge

  8. KSP_K560GFP

    Plasmid
    #129770
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129770-plasmid-map-sequence-id-263613

    Enlarge

  9. pIVTRup

    Plasmid
    #101362
    Purpose
    Highly efficient in vitro transcription. Empty backbone.
    Depositor
    Ángel Raya
    Insert
    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    Use
    For cloning a gene for in vitro transcription pur…
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    101362-plasmid-map-sequence-id-352564

    Enlarge

  14. CDK1as_T2A_Zeo

    Plasmid
    #118596
    Purpose
    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.
    Depositor
    William Earnshaw
    Article
    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.
    Insert
    CDK1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    118596-plasmid-map-sequence-id-229650

    Enlarge

  15. pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

    Plasmid
    #105127
    Purpose
    Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein
    Depositor
    Didier Stainier, Kimberley Evason
    Article
    Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d
    Inserts
    Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    cryaa (crystallin) and fabp10a
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    105127-plasmid-map-sequence-id-220518

    Enlarge

  20. XE57 Delta N-cadherin

    Plasmid
    #17025
    Depositor
    Randall Moon
    Insert
    dominant N-cadherin
    Use
    Xenopus expression
    Tags
    Expression
    Mutation
    nt 90-2004 deleted
    Promoter
    Available since
    April 15, 2008
    Availability
    Academic Institutions and Nonprofits only
    17025-plasmid-map-sequence-id-337144

    Enlarge

Showing: 1 - 20 of 365 results