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Showing: 1961 - 1980 of 47545 results

  1. pAAV asyn S129A

    Plasmid
    #36066
    Depositor
    Hilal Lashuel, Patrick Aebischer
    Insert
    alpha-synuclein S129A (SNCA Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S129A
    Promoter
    CMV
    Available since
    Sept. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  2. pUPD2 PhyB (GB2721)

    Plasmid
    #160603
    Purpose
    chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.
    Depositor
    Diego Orzaez
    Insert
    PhyB
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160603-plasmid-map-sequence-id-322052

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  3. SDCBPA-c000

    Plasmid
    #175480
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-V288.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175480-plasmid-map-sequence-id-353167

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  4. Z-AIMP1-1

    Plasmid
    #166560
    Purpose
    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166560-plasmid-map-sequence-id-329268

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  5. pAAV asyn S129D

    Plasmid
    #36069
    Depositor
    Hilal Lashuel, Patrick Aebischer
    Insert
    alpha-synuclein S129D (SNCA Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S129D
    Promoter
    CMV
    Available since
    Sept. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  6. APEX2 VLP

    Plasmid
    #182211
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-APEX2
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology; Tem imaging
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182211-plasmid-map-sequence-id-357716

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  7. integrin beta6 Y774F pBabe puro

    Plasmid
    #13599
    Depositor
    Dean Sheppard
    Insert
    integrin beta 6 Y774F (ITGB6 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Y774F
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  8. pREST-donor

    Plasmid
    #113807
    Purpose
    CRISPR donor plasmid to tag human transcription factor REST with GFP
    Depositor
    Kevin White
    Insert
    REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs4864608
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113807-plasmid-map-sequence-id-231037

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  9. p-dCas9-p300-S1396R-S1397R-Hygro

    Plasmid
    #104419
    Purpose
    transient expression of dCas9-dp300 fusion protein
    Depositor
    Stephan Beck
    Insert
    dp300
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    S1396R-S1397R
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104419-plasmid-map-sequence-id-204303

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  10. AAV Ef1a-mRuby3-WPRE

    Plasmid
    #135427
    Purpose
    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.
    Depositor
    Rylan Larsen
    Insert
    mRuby3
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135427-plasmid-map-sequence-id-271155

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  11. H2B-sfGFP-T2A-iCre

    Plasmid
    #178546
    Purpose
    pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox and Mouse Targeting; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178546-plasmid-map-sequence-id-360157

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  12. O-IL8-15

    Plasmid
    #166544
    Purpose
    scFv of a human scaffold targeting Interleukin-8. Antigen coverage aa 28-99 of 99, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166544-plasmid-map-sequence-id-328277

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  13. pLV H2afy-V5

    Plasmid
    #175122
    Purpose
    Lentiviral expression of mouse Macroh2a1-V5
    Depositor
    Larry Gerace
    Insert
    Macroh2a1 (Macroh2a1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175122-plasmid-map-sequence-id-351930

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  14. integrin alpha9 EGFP pBabe puro

    Plasmid
    #13605
    Depositor
    Dean Sheppard
    Insert
    integrin alpha 9 (ITGA9 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  15. cTRE-PtenWT

    Plasmid
    #135663
    Purpose
    Introduce Dox-inducible (TRE promoter) wildtype Pten cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    135663-plasmid-map-sequence-id-325426

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  16. pGEX4T-1-Nix

    Plasmid
    #100759
    Purpose
    Bacterial expression of GST fused Nix protein
    Depositor
    Joseph Gordon
    Insert
    GST-Nix (BNIP3L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100759-plasmid-map-sequence-id-193048

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  17. pTEAD1-donor

    Plasmid
    #112329
    Purpose
    CRISPR donor plasmid to tag human transcription factor TEAD1 with GFP
    Depositor
    Kevin White
    Insert
    TEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112329-plasmid-map-sequence-id-228556

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  18. pUSF2-donor

    Plasmid
    #112361
    Purpose
    CRISPR donor plasmid to tag human transcription factor USF2 with GFP
    Depositor
    Kevin White
    Insert
    USF2 homology arms flanking EGFP-IRES-Neo cassette (USF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:35278400:T>A, hg38:19:35278455:G>C
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112361-plasmid-map-sequence-id-229684

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  19. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104407-plasmid-map-sequence-id-204449

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  20. p14-9VNA5

    Plasmid
    #50931
    Purpose
    Expresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 96-100 of SRP14 changed to alanine
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1961 - 1980 of 47545 results