Addgene

Showing: 1961 - 1980 of 3755 results

Results per page:

Sort results by:

pCMV HA hRb delta CDK + S230

Plasmid

#58907

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S230 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T252

Plasmid

#58910

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T252 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV superYFP-AURKA K162M-mTurq2

Plasmid

#157773

Purpose

Expression of AuroraA kinase-dead biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

mTurquoise2 and superYFP

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Flag-YAP-S127/387A

Plasmid

#33079

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, S387A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa452-526

Plasmid

#73381

Purpose

Expresses amino acids 452-526 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 452-526 of XPLN (arhgef3) p…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa304-466 (PH)

Plasmid

#73380

Purpose

Expresses amino acids 304-466 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 304-466 of XPLN (arhgef3) p…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa107-311

Plasmid

#73379

Purpose

Expresses amino acids 107-317 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 107-317 of XPLN (arhgef3) p…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV4-Flag-mIL-17RA/CFP.Hygro

Plasmid

#46861

Purpose

encodes Flag-tagged mouse IL-17RA fused to Cyan Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-CFP

Use

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pCMV Lex VP16 (F442A) HA (P#1710)

Plasmid

#14595

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA and VP16 (F442A)

Expression

Mammalian

Mutation

The VP16 sequence contains an F442A mutation. The…

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

Plasmid

#58414

Purpose

Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA internal

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine; The sequenc…

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-CHD5-Y619E-FRAG

Plasmid

#68871

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa, Y619E

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-CHD5-WT-331-672aa

Plasmid

#68870

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa)

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCMV:: NES-FKBP-iLID-LRP6c(△1-64)

Plasmid

#221775

Purpose

Expresses NES-FKBP-iLID-LRP6c(△1-64) component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: NES-FKBP-iLID-LRP6c

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV:: NES-FKBP-iLID-LRP6c

Plasmid

#221774

Purpose

Expresses NES-FKBP-iLID-LRP6c component in mammalian cells

Depositor

Dongmin Lee

Article

Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

Insert

pCMV:: NES-FKBP-iLID-LRP6c

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DNAJC5-ΔC30 (Δ169-198)

Plasmid

#205722

Purpose

Mammalian expression of DNAJC5-ΔC30 (Δ169-198)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

Δ169-198

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mScarlet-I-eDHFR-ORP5(ΔPH)

Plasmid

#214277

Purpose

Mammalian expression of ORP5(ΔPH) fused to mScarlet-I-tagged E. coli DHFR

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chemical Biology

Insert

mScarlet-I-eDHFR-ORP5(253-879) (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-XPACK-DNAJC5 (L115R)- HaloTag

Plasmid

#205712

Purpose

Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag and XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DEAD XPACK-DNAJC5 (L115R)

Plasmid

#205718

Purpose

Mammalian expression of DEAD XPACK-DNAJC5 (L115R)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Dead XPACK (GCINIKRKD)

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DNAJC5-ΔC10 (Δ189-198)

Plasmid

#205720

Purpose

Mammalian expression of DNAJC5-ΔC10 (Δ189-198)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

Δ189-198

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DNAJC5-ΔC20 (Δ179-198)

Plasmid

#205721

Purpose

Mammalian expression of DNAJC5-ΔC20 (Δ179-198)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

Expression

Mammalian

Mutation

Δ179-198

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge