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Plasmid#185012PurposeBacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion ProteinDepositorInsertCBX5 (HP1a) (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3-WPRE
Plasmid#132318Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.iAChSnFR
Plasmid#137955PurposeExpresses iAChSnFR under CAG promoterDepositorArticleHas ServiceAAV1 and AAV9InsertiAChSnFR
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP3Kv-WPRE
Plasmid#132332Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vectorDepositorInsertASAP3Kv
UseAAVTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-Sox10
Plasmid#115242PurposeLentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)DepositorInsertSOX10 (SOX10 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iAChSnFR
Plasmid#137950PurposeExpresses iAChSnFR under hSynapsin promoterDepositorArticleHas ServiceAAV1InsertiAChSnFR
UseAAVTagsExpressionMammalianMutationPromoterhSynanpsinAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCROPseq-VEX-optimized-scaffold
Plasmid#203321PurposeModified CROPseq vector for efficient CRISPR screens in hematopoiesisDepositorInsertsgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4-AARE-luc2P-Hygro
Plasmid#101787PurposeLuferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)DepositorInsert3xAARE
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.ZAC.GFAP.iAChSnFR
Plasmid#137962PurposeExpresses iAChSnFR under GFAP promoterDepositorArticleInsertiAChSnFR
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ M2rtTA_CAGG TetON-Sox10
Plasmid#115240PurposeRMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)DepositorInsertSOX10 (SOX10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CNTF-HA
Plasmid#195517PurposeExpresses HA-tagged CNTF in mammalian cellsDepositorInsertCiliary neurotrophic factor (CNTF Human)
UseAAVTagsHA-tag and NGF signal peptideExpressionMammalianMutationPromoterUbC promoter with beta-globin intronAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iAChSnFR-NULL
Plasmid#137951PurposeExpresses iAChSnFR (non-binding) under hSynapsin promoterDepositorArticleInsertiAChSnFR (non-binding variant)
UseAAVTagsExpressionMammalianMutationY140APromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTF-ACE2s
Plasmid#162786PurposeThe extracellular domain of Angiotensin converting enzyme 2 (ACE2) expressionDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTags10xHis, FLAG, and hTPA leaderExpressionMammalianMutationPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-PHD2-181-426
Plasmid#223551PurposeHis-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purificationDepositorInsertPHD2 (EGLN1 Human)
UseTags6x HisExpressionBacterialMutationDeleted amino acids 1-180.PromotertrcAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(FOXH1_5-2)-PGKpuroBFP-W
Plasmid#211962PurposeExpress gRNA against FOXH1 with puro and BFPDepositorInsertsgRNA targeting FOXH1 (FOXH1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(FOXH1_5-4)-PGKpuroBFP-W
Plasmid#211963PurposeExpress gRNA against FOXH1 with puro and BFPDepositorInsertsgRNA targeting FOXH1 (FOXH1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHsSyx3-MBP
Plasmid#179286PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6DepositorInsertSyx
UseTagsTEV protease cleavage site + mature maltose bindi…ExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HsSyx3
Plasmid#179287PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + mature maltose binding protein + TEV prote…ExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HsSyx-375-792
Plasmid#179291PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + mature maltose binding protein + TEV prote…ExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus2-Y705F/S727A-STAT3
Plasmid#123177PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
UseTagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationDouble Y705F and S727A substitutionsPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only