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Plasmid#105780PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsmRuby3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only
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pDONR223-ZNF770
Plasmid#88562PurposeDonor Vector containing ZNF770 transcription factor, part of the Human TFome CollectionDepositorInsertZNF770 (ZNF770 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mRuby3
Plasmid#105797PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mRuby3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK mCh-V5-NES-EGFP-ERBB2 NLS zeo
Plasmid#192338PurposeLentiviral expression vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLSDepositorInsertmCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-TuSC
Plasmid#178079PurposeInsect cell expression of gamma-TuSCDepositorUseTagsTEV-His6ExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
FENEF-Sensor
Plasmid#208930Purposein vitro p38-Sensor for luminescent detection of p38-activityDepositorInsertFENEF sequence from human ATF2 with phospho site (ATF2 Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ZKSCAN5_SMO
Plasmid#205960PurposeExpress mEGFP-tagged fusion protein, ZKSCAN5_SMO from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-AKT1
Plasmid#170682Purposedoxycycline-inducible overexpression of AKT1DepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-HAND1
Plasmid#170689Purposedoxycycline-inducible overexpression of HAND1DepositorInsertHAND1 (HAND1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-ERBB2 NLS
Plasmid#192316PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLSDepositorInsertmCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-HAND2
Plasmid#170690Purposedoxycycline-inducible overexpression of HAND2DepositorInsertHAND2 (HAND2 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-NR2F1
Plasmid#170698Purposedoxycycline-inducible overexpression of NR2F1DepositorInsertnuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationdeletion of 36G- please see depositor commentsPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11
Plasmid#161923PurposeTo generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.DepositorInsertsgRNA targeting HSD17B11 exon 1
UseCRISPRTagsExpressionMutationPromoterU6Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
tetO-HEY2
Plasmid#170691Purposedoxycycline-inducible overexpression of HEY2DepositorInsertHEY2 – hes related family bHLH transcription factor with YRPW motif 2 (HEY2 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-RDH11
Plasmid#161924PurposeTo generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.DepositorInsertsgRNA targeting RDH11 exon 2
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-FGF11 [N92/14R]
Plasmid#206602PurposeMammalian Expression Plasmid of anti-FGF11 (Human). Derived from hybridoma N92/14.DepositorInsertanti-FGF11 (Homo sapiens) recombinant Mouse monoclonal antibody (FGF11 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-ALN
Plasmid#43918DepositorUseLentiviral; Doxycycline inducibleTags6xHis and V5ExpressionMammalianMutationGylcine-serine-glycine (GSG) linker followed by v…PromoterTRE promoter, Tet-ONAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only