-
Plasmid#84034PurposeContains Golden Gate cassette to express up to four gRNAs and dsREDDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only
-
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
UseTagsHis14 and bdNEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 236HSC WT (BRCA2)
Plasmid#16246DepositorInsertBRCA2 (BRCA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SV40 LT
Plasmid#13970DepositorInsertSV40 LT (SV40gp6 )
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)
Plasmid#179116PurposeCargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site for in-frame fusion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Luciferase
Plasmid#21375Purposeself-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and LuciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
phCMV-coGALVenv-C4070A
Plasmid#172217PurposeExpresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectorsDepositorInsertEnvelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
UseTagsExpressionMammalianMutationCarries C-terminus of amphotropic MLV strain 4070…PromoterCMV immediate early promoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
mIL12-N1
Plasmid#123139PurposeMammalian expression of murine cytokine IL-12 under CMV promoterDepositorInsertMurine IL12 tandem p40 p35
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-H1047R
Plasmid#116500PurposeLentiviral expression of PIK3CA H1047RDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationH1047RPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-UBE2E1 WT in pcDNA3.1
Plasmid#118213PurposeExpresses HA-UBE2E1 in mammalian cellsDepositorInsertUBE2E1 (UBE2E1 Human)
UseTagsHAExpressionMammalianMutationnonePromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dazl-CPG-TV
Plasmid#70144Purposea targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyADepositorInsert5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
UseMouse Targeting and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
PB-tetO-NB
Plasmid#197092PurposePiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiationDepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKL123
Plasmid#187889PurposeAAVS1 safe-harbor integration of dox-inducible NFIA-SOX9DepositorInsertNFIA-SOX9
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PHIS3-GFP-μNS
Plasmid#116935PurposeYeast integrative vector for the expression of μNSDepositorInsertμNS
UseTagsGFPExpressionYeastMutationPromoterHIS3Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN3-5ht6
Plasmid#35624DepositorInsert5ht6 (Htr6 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorInsertSrc biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
phCMV-GALV-MTR
Plasmid#163612PurposeRetro and Lentiviral transduction of primary human germinal center B cellsDepositorInsertGaLV MTR envelope
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CR4
Plasmid#62655PurposeCas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetRDepositorInsertCas9 nuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ATF6
Plasmid#32955PurposeFor visualization of GFP-ATF6 in mammalian cells.DepositorInsertATF6 (ATF6 Human)
UseTagsEGFPExpressionMammalianMutationM67L and M313IPromoterCMVAvailable sinceNov. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RPS6 (pDD1902)
Plasmid#52913Purposecontains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.DepositorInsertHAS6 (RPS6 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
PGKp-GFP-Empty
Plasmid#174175PurposeControl lentiviral vector expressing GFP under control of the human PGK promoter.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Syn_CapChR2_eYFP
Plasmid#188035PurposeExpresses CapChR2-eYFP in mammalian neuronsDepositorInsertCapChR2-eYFP
UseAAVTagseYFPExpressionMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Tet1CD-P2A-BFP
Plasmid#108245PurposeLentiviral vector to express dCas9-Tet1CD-P2A-tagBFPDepositorInsertdCas9-Tet1CD-HA-P2A-BFP
UseLentiviralTagsHAExpressionMammalianMutationdCas9-D10A, dCas9-H840APromoterUbcAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218633PurposeProximity biotinylation of msNLRP3DepositorInsertNLRP3 (Nlrp3 Mouse)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
GW1-PercevalHR
Plasmid#49082PurposeMammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.DepositorInsertPercevalHR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
AGTR1-Tango
Plasmid#66222PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertAGTR1 (AGTR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-cyto-iATPSnFR1.0
Plasmid#102553Purposeexpresses cytoplasmic ATP sensor in astrocytesDepositorInsertiATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs GFP-LC3-RFP
Plasmid#117413PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PE-Max-pet21A
Plasmid#204471PurposeExpression plasmid for PE protein in bacteria.DepositorInsertPrime editor
UseCRISPRTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-FLEX-BFP2-(mCherry)’
Plasmid#115234PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after itDepositorInsertmTagBFP2 and mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage-ubc-nls-ha-tdMCP-gfp
Plasmid#40649DepositorInserttdMCP-gfp
UseLentiviralTagsEGFP, HA, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL4-ERSE2-luc2P-Hygro
Plasmid#101790PurposeLuciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)DepositorInsert3xERSE-II
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry Vinculin
Plasmid#50527DepositorInsertVinculin (VCL Chicken)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
Plasmid#202566PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopyDepositorInsertLINE-1
UseTagsmNeonGreen2 on ORF1pExpressionMammalianMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CAPN1
Plasmid#60941PurposeExpresses calpain1 in mammalian cellsDepositorInsertCalpain1 (CAPN1 Human)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-R203M
Plasmid#177952PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (R203M) (N )
UseTagsExpressionMammalianMutationR203MPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CreEGFP
Plasmid#165140PurposeExpression of Cre recombinase fused with EGFP in C-terminalDepositorInsertCre recombinase
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only