We narrowed to 8,610 results for: plat
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Plasmid#227325PurposeDonor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)DepositorInsertGOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMC00028 (aka pTS0057)
Plasmid#200832PurposeAAVS1 guide RNA cloned into the lentiSAM puro backboneDepositorInsertAAVS1 guide RNA
UseLentiviralExpressionMammalianAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-H2B-tdTomato-SRT
Plasmid#203393PurposeAAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cardsDepositorInsertH2B fused piggyBac tdTomato self-reporting transposon
UseAAVTags3X SV40 and H2BExpressionMammalianPromoterEf1aAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0691
Plasmid#142214PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG1-srt-his
Plasmid#124802PurposeHDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.DepositorInsertMurine IgG1
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianPromoter-Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-V12
Plasmid#125136PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).DepositorInsertmNeon/mScarletP2A Rac1-V12
ExpressionMammalianMutationRemoved Not1 site in mScarlet. 12th AA in Rac1 ch…PromoterCAGAvailable SinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI
Plasmid#115220PurposeExpresses Pin3Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin3Cas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI
Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry
Plasmid#107246PurposehM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.DepositorArticleInserthM3DREADD
UseAAVTagsABIcs, FLAG, and mCherryMutationT to D and S to D mutations in V2-tailPromoterhSYNAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus-TmiR-G13-G12
Plasmid#25742PurposeLentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.DepositorInsertG alpha 13 and 12 miR-shRNA
UseLentiviral and RNAiTagsVenusExpressionMammalianAvailable SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_h7SK
Plasmid#102846PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_h7SK
UsePcr template for stagr insertsPromoterH7SKAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB2_IRES_EGFP
Plasmid#223506PurposeExpression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0267
Plasmid#142502PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
KM-CashGem-Xyl2-URA3-LINEAR
Plasmid#174838PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianusDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterTEF1pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
JL202: pMVP/mCherry-DEST
Plasmid#121853PurposepMVP destination vector, empty expression vector backbone w/ mCherry selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L
Plasmid#121833PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/Dnmt3a-3L (open)
UseGateway Cloning and Synthetic BiologyTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CCL5.Pro
Plasmid#79492PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoterDepositorAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Dnmt3b1-Poly(A)-PuroR
Plasmid#65556PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 1Available SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 AK3 Hyg
Plasmid#37539DepositorAvailable SinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only