-
Plasmid#44744DepositorInsertp65 (Rela Mouse)
UseTagsExpressionBacterialMutationAA 19-325PromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del2 pET28a
Plasmid#122116PurposeExpression Brg1 amino acids 1-837 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTags6XHis and His-T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Sangamo-HIS
Plasmid#40787Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsC Terminal 6xHISExpressionBacterialMutation+63 C Terminus,Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-HpENR
Plasmid#12651DepositorInsertnoyl-acyl carrier protein reductase (fabI Helicobacter pylori)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-ChE1 v4.5
Plasmid#185755PurposeBacterial expression of ChE1 v4.5DepositorInsertChE1 v4.5
UseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Himar-dCas9
Plasmid#137079PurposeHimar-dCas9 gene with C-terminal 6x-His tag for protein purificationDepositorInsertHimar-dCas9
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-2HMG-eGFP
Plasmid#112737PurposeExpress 2HMG-eGFP in E. coliDepositorInsert2HMG-eGFP
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo3
Plasmid#25103DepositorInsertpro-SUMO3 (SUMO3 Human)
UseTagsHisExpressionBacterialMutationchanged Serine 2 to AlaninePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3991 pET ubcH9
Plasmid#8651DepositorInsertUbcH9 (UBE2I Human)
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB3m
Plasmid#120289PurposeProduce human recombinant mTGFB3 in DE3 cellsDepositorInsertmonomeric Transforming Growth Factor-β3
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-YTHDF2
Plasmid#204406PurposeE. coli expression of full-length YTHDF2 with an N-terminal mCherry tagDepositorInsertYTHDF2 (YTHDF2 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i2
Plasmid#120883PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.DepositorInsertCas12i2
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delWGR-L713F
Plasmid#173941PurposeBacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutationDepositorInsertPARP1delWGR-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 521-660; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB1m
Plasmid#120286PurposeProduce human recombinant mTGFB1 in DE3 cellsDepositorInsertmonomeric Transforming Growth Factor-β1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromotorAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_0143-pET30-Fusicatenibacter_saccharivorans
Plasmid#116952PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL15-His6
Plasmid#201880PurposeUsed for bacterial expression of human protein ARL15DepositorInsertARL15 (ARL15 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
BioBrick pET-24a(+)
Plasmid#117802PurposeBioBrick pET vector for protein overexpression in E. coliDepositorTypeEmpty backboneUseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
UseTags6His-TEVExpressionBacterialMutationR49APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only