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pCMV 3xFLAG-LC3B Q116P G120

Plasmid

#129289

Purpose

Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Changed Glutamine 116 to Proline. Deleted amino a…

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV4-Flag-mIL-17RA/YFP.Hygro

Plasmid

#46862

Purpose

encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-YFP

Use

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T826

Plasmid

#58921

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T826 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Tag/WT SPAST without 5'UTR

Plasmid

#87732

Purpose

Expresses WT spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

5'UTR deleted (1-190 bp)

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + S249

Plasmid

#58908

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S249 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

Plasmid

#115139

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3.1 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pVH227-Tier1-OTetO7-PCMVmin2-SEAP

Plasmid

#169563

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP production

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + S612

Plasmid

#58914

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S612 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag p38 alpha(agf)

Plasmid

#20787

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced Thr180 and Tyr182 with Ala and Phe, resp…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4D-CD4-FLAG

Plasmid

#51601

Purpose

expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Use

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S608

Plasmid

#58913

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S608 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S127/384A

Plasmid

#33076

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, S384A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

Plasmid

#134332

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Lmo

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

Plasmid

#134334

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA17_Sga

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

Plasmid

#134335

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sma

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

Plasmid

#134336

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Ssim

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)

Plasmid

#134338

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sga

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

Plasmid

#134339

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Spse

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge