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pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

Plasmid

#162230

Purpose

Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-human MYCN-EF1-mRFP

Plasmid

#193708

Purpose

Lentiviral expression of human MYCN

Depositor

Martine Roussel

Insert

MYCN (MYCN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

Plasmid

#90003

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

Plasmid

#90004

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-human-c-MYC-ER-mouse IRES-mcherry

Plasmid

#73570

Purpose

Mammalian retroviral expression of Myc-ER

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMYC (MYC Human)

Use

Retroviral

Tags

IRES-mCherry and Mouse ER

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCXLE-Sox2-T2A-Klf4-E2A-Myc

Plasmid

#210018

Purpose

episomal SKM (expressing mouse Sox2 + Klf4 + Myc) for integration-free naïve reset

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

Plasmid

#133028

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Golgin84 (GOLGA5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-673

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

Plasmid

#134864

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

emerin (EMD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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loxP-myctagBFP-STOP-loxP-mCherry-NTR

Plasmid

#108456

Purpose

This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

loxP-myctagBFP-STOP-loxP-mCherry-NTR

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLINK cyclin T1 (1-280) myc (K93D) (P#580)

Plasmid

#14611

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) K93D (CCNT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

K93D. Deficient in binding to CDK9. Inactive in 3…

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

Plasmid

#134863

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

Plasmid

#71811

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-Myc-gate-pGK-HYG

Plasmid

#107395

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro HEXIM1

Plasmid

#200726

Purpose

Lentiviral expression of Flag-Tagged human HEXIM1

Depositor

Claudio Alarcón

Insert

HEXIM1 (HEXIM1 Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-GFP-EWSR1-myc-His

Plasmid

#46385

Purpose

expresses EGFP tagged EWSR1 in mammalian cells

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Tags

EGFP, His, and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEF myc Gal cyclin T1 (1-726) (P#1026)

Plasmid

#14626

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

cyclin T1 (CCNT1 Human)

Use

Tags

Gal and myc

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

Plasmid

#90008

Purpose

Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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