-
Plasmid#222998PurposeMCS reporter constitutively expressing EGFP and conditionally expressing a synthetic cell surface protein upon prime editing. For lentivirus packaging.DepositorInsertIGKleader-hIgG1_FC-Myc-PDGFRb-IRES2-EGFP
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
Plasmid#222742PurposeCaST-IRES for neuron expressionDepositorInsertCD4-sTC-M13-GFP-IRES-CaM-V5-sTN
UseTagsHA and V5ExpressionMammalianMutationPromoterSynapsinAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)
Plasmid#209078PurposeNgn2 mediated cortical neuron induction, along with SNCA(3K) over-expressionDepositorUseTagssfGFPExpressionMammalianMutation3K mutant: E35K, E46K, E61KPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)
Plasmid#229839PurposeA CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.DepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
Plasmid#219782PurposeCaST-IRES for HEK cell expressionDepositorInsertCD4-sTC-M13-GFP-IRES-CaM-V5-sTN
UseTagsHA and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag
Plasmid#216696PurposeSynchronize trafficking of TNFalpha from the ER (RUCH system)DepositorInsertsFIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro
Plasmid#141371PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationC145A, human codon optimizedPromoterEF1alphaAvailable sinceApril 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP
Plasmid#32477PurposeCre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP markerDepositorInsertPSAML141F,Y115F-5HT3HC
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S
Plasmid#62460Purposeexpresses C151S and C288S double mutant human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationChanged Cysteines 151 and 288 of Keap1 to respect…PromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro
Plasmid#167832PurposeInducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLSDepositorInsertHRAS(N17) (HRAS Human)
UseLentiviralTagsExpressionMammalianMutationS17NPromoterUbc, TREAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE
Plasmid#89587PurposeFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.DepositorInsertnuclear localization signal (nls) turboRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP
Plasmid#32475PurposeCre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP markerDepositorInsertPSAMQ79G,Q139G-5HT3HC
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceDec. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B mCherry reporter
Plasmid#68714DepositorInsertH2B mCherry and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP
Plasmid#18808DepositorInsertChx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)
Plasmid#139444PurposeLentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4DepositorInsertFLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationsequence codon-optimizedPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro
Plasmid#89994PurposeDonor template for generation of NGN3-ntdTomato reporter cell linesDepositorInsertNGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry
Plasmid#122959PurposeLentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expressionDepositorInsertNOTCH2NL-delta C terminus (NOTCH2NLB Human)
UseLentiviralTags6xmyc tagExpressionMutationdeletion of C terminus from full length NOTCH2NLBPromoterCAG promoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP
Plasmid#122956PurposeLentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expressionDepositorInsertNOTCH2NL-delta C terminus (NOTCH2NLB Human)
UseLentiviralTags6xhis tagExpressionMutationdeletion of C terminus from full length NOTCH2NLBPromoterCAG promoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9
Plasmid#231910PurposeFor packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only