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pAAV.hSyn-FLEX.iGABASnFR.F102Y.Y137L

Plasmid

#112165

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Tags

Expression

Mutation

F102Y.Y137L

Promoter

Synapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMflCT-o4

Plasmid

#101313

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMflT-o1

Plasmid

#101309

Purpose

Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pClneoEGFPC-let60 K16N

Plasmid

#66861

Purpose

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 16 is mutated to asparagine

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E/R61E

Plasmid

#60354

Purpose

R53E R61E double mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E and R61E mutations; contains amino acids 7-1…

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E222A-E272A

Plasmid

#35060

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; E222A and E272A…

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-Y218A-Y267A

Plasmid

#35059

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; Y218A and Y267A…

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E381A

Plasmid

#35058

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; E381A mutation

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E272A

Plasmid

#35057

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; E272A mutation

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E222A-E272A-E381A

Plasmid

#35061

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; E222A, E272A, a…

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E222A

Plasmid

#35056

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; E222A mutation

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-T220A

Plasmid

#35055

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; T220A mutation

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-Y218A

Plasmid

#35054

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; Y218A mutation

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pTH394-SUP45-L34S

Plasmid

#29373

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

L34S mutant gene (corresponding to the sup45-36ts…

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pTH401-SUP45-R65C

Plasmid

#29381

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

R65C mutant gene (corresponding to the sup45-3 al…

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_NR2F2_Hormone-recep

Plasmid

#109826

Purpose

Protein expression and purification of NR2F2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR2F2_Hormone-recep (NR2F2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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mCardinal-N1

Plasmid

#54590

Purpose

Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-4T-1 3xFlag-MAPKAPK2

Plasmid

#82720

Purpose

Expresses human MAPKAPK2

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MAPKAPK2 (MAPKAPK2 Human)

Use

Tags

3xFLAG and GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pET28C-mCherry-MED1LCD

Plasmid

#204404

Purpose

E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

MED1(947-1581) (MED1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

Plasmid

#138610

Purpose

Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Inserts

Hyperactive AID
T7 RNA Polymerase
tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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