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Plasmid#36877DepositorInsertGFP
UseTagsExpressionMammalianMutationF64L; S65T; V163A; S175GPromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTomatoSlicer
Plasmid#221085PurposeDual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GST-EGFP-GPIDAF
Plasmid#213722PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsV5-tag-GSTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GST-EGFP-GPICEAM7
Plasmid#213723PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsV5-tag-GSTExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-NLS-ZapCV2-ALA
Plasmid#203665PurposeExpresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cellsDepositorInsertZapCV2-Ala zinc dead sensor
UseTagsSV40 NLSExpressionMammalianMutationCys and His in Zap1 is mutated with AlaPromoterCMV promoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP humanAPOBEC1 L173A/G227A
Plasmid#167171PurposeExpresses human APOBEC1 L173A/G227A in mammalian cellsDepositorArticleInserthumanAPOBEC1 (APOBEC1 Human)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3)
Plasmid#182369PurposeDendra2-based translation reporterDepositorInsertmtIF3 (Mtif3 Mouse)
UseTagsPal-Dendra2ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_V577D-talin1 (1-2541)
Plasmid#166115Purposeexpresses talin-1-V577D in mammalian cellsDepositorInsertV577D-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_P229L-talin1 (1-2541)
Plasmid#166113Purposeexpresses talin-1-P229L in mammalian cellsDepositorInsertP229L-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTNG5kwh
Plasmid#128279PurposeMammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion proteinDepositorInserthTetR::NLS::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsEGFPExpressionMammalianMutationPromoterCMV-D2ir promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP773_pLenti-6xHRBKET-sgRNA
Plasmid#211766PurposeMultiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selectionDepositorInsertgRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
UseLentiviralTagsExpressionMutationPromoterpCMV and U6Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRIGF-IKZF3
Plasmid#69046Purposeexpression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorInsertIKZF3 (IKZF3 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterIRESAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRF.1 udsVenus (P_JUND)
Plasmid#58692PurposeA rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)DepositorInsertendogenous promoter of JUND (JUND Human)
UseLentiviralTagsudsVenusExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVtet1_bGHpA
Plasmid#177357PurposeAAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterTetracycline-dependent promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-scFVtet2_bGHpA
Plasmid#177353PurposeAAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 2 (TET2 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationCatalytic domains of human TET2 (1129–1936 and 14…Promoterhuman Synapsine promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg3
Plasmid#127160PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotypeDepositorInsertIghg3 (Ighg3 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla
Plasmid#199551PurposePiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoterDepositorUsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterTRE+CMVmin promoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TERT - WT
Plasmid#213827PurposeExpresses human telomerase reverse transcriptase, the catalytic subunit of telomeraseDepositorInserthuman TERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ISDra2-TnpB-mCherry-U6-ωRNA*
Plasmid#212967PurposeExpression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoterDepositorInsertsISDra2-TnpB-T2A-mCherry
ωRNA*
UseCRISPRTagsNLS and NLS-FLAGExpressionMammalianMutationPromoterCAG, T7 and U6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
TERT - R865C
Plasmid#213926PurposeExpresses Mutant TERT R865CDepositorInsertMutant TERT R865C (TERT Human)
UseLentiviralTags6x His TagExpressionMammalianMutationArginine 865 changed to CysteinePromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only