Addgene

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Showing: 1921 - 1940 of 9036 results

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pFOSGE_COF2D05

Plasmid

#70892

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

dbo (dbo Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2G06

Plasmid

#70900

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED17 (MED17 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2H07

Plasmid

#70908

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG7179 (CG7179 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2H09

Plasmid

#70924

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Mnn1 (Mnn1 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
MXS_PA-GFP

Plasmid

#62412

Purpose

MXS_chaining vector with PA-GFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PA-GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_loxP

Plasmid

#62430

Purpose

MXS_chaining vector with loxP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

loxP site

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_linker

Plasmid

#62431

Purpose

MXS_chaining vector with linker

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Linker 12AA Gly-/Ser-rich

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_MCS4

Plasmid

#62398

Purpose

MXS_chaining vector with MCS4

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

MluI-XhoI-PvuII-BglII-SalI MCS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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M26L DJ-1 pET15b

Plasmid

#60679

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26L DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26L

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pDS69

Plasmid

#48367

Purpose

FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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psiCheck2 6xmut UTR

Plasmid

#40764

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

6 copies (3 site expanded) of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

TK/SV40

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-mCherry-flex-dtA

Plasmid

#58536

Purpose

Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

Depositor

Naoshige Uchida

Article

Wu et al Nature. 2014 May 15;509(7500):325-30. do

Insert

dtA (DIP_RS18250 )

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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psPax2-D64V-NC-mNeon

Plasmid

#196509

Purpose

mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

mNeon

Use

Tags

mNeon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPEG

Plasmid

#162466

Purpose

Entry vector for gRNA insertion in plant prime editing expression vector

Depositor

Adam Bogdanove

Article

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Type

Empty backbone

Use

Entry vector for grna

Tags

Expression

Mutation

Promoter

CmYLCV

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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scFv-IFP2.0N

Plasmid

#215746

Purpose

Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0N

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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H3K9me3Probe_FullLength

Plasmid

#186463

Purpose

Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

Depositor

Chongli Yuan

Article

Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

Insert

dimeric CDY1 (CDY1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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Dimer cleaved

Plasmid

#198199

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-T2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

A206K both

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 Chfr (1014)

Plasmid

#61853

Purpose

mammalian expresion of Chfr fused to EGFP

Depositor

Jonathon Pines

Article

Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

Insert

Chfr (CHFR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
mCherry-VP40

Plasmid

#74421

Purpose

Mammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusion

Depositor

Judith White

Article

Shoemaker et al PLoS One. 2013;8(2):e56265. doi: 10.1371

Insert

VP40

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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