-
Plasmid#111858PurposeN-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, SNAPf-tag, TEV site, and ZZExpressionInsectMutationR250Q, Optimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Sec-APEX2m
Plasmid#176844PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-Sec-APEX2m
UseInducibleTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAT
Plasmid#187416PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorUseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1-GFP
Plasmid#111657PurposeC-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1-GFP (Bicdl1 Mouse)
UseTags8xHis-tag, GFP, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)
Plasmid#70668PurposeHuman mutant of E2F1 lacking amino acids 2 to 126DepositorInsertE2F1 lacking residues 2 to 126 (E2F1 Human)
UseTagsHAExpressionMammalianMutationlacks amino acids 2-126PromotercmvAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mGreenLantern
Plasmid#164467PurposeVisualization of the ER in cells (compatible with OSER assay)DepositorInsertmGreenLantern
UseTagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-CIB1-dCas9
Plasmid#63665PurposeMammalian gRNA expression vector also expressing CIB1-dCas9DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 and CMVAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 C1(259-443)
Plasmid#89832PurposeExpress GATA3 C1 in mammalian cellsDepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsHAExpressionMutationC1 (AA 259-443)PromoterCMVAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDN(1-400)-GFP
Plasmid#111862PurposeC-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDN(1-400)-GFP (Bicd2 Mouse)
UseTags8xHis tag, GFP, and ZZ tagExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-SNAPf-Psc-StrepII
Plasmid#111860PurposeC-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.DepositorInsertHook3(1-552)-SNAPf (HOOK3 Human)
UseTagsPsc, SNAPf, and Strep-IIExpressionInsectMutationOptimised for expression in Sf9 cellsPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-GFP-Psc-StrepII
Plasmid#111861PurposeC-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.DepositorInsertHook3(1-552)-GFP (HOOK3 Human)
UseTagsGFP, Psc tag, and Strep tagExpressionInsectMutationOptimised for expression in SF9 cellsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorInsertPHD2 (EGLN1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HIS_TEV_hsSYX3
Plasmid#179290PurposeE. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7lacAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 L211P
Plasmid#124487Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged leucine 211 to prolinePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 R312H
Plasmid#124488Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged arginine 312 to histidinePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 G381R
Plasmid#124490Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged glycine at 381 to argininePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 F416L
Plasmid#124491Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged phenyalanine 416 to leucinePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 G379R
Plasmid#124489Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged glycine at 379 to argininePromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP3-WPRE
Plasmid#132331Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vectorDepositorInsertASAP3
UseAAVTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only