RAT
(Plasmid #187416)

• Purpose: Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
• Depositing Lab: Susanne Dobler
• Publication: Mohammadi et al bioRxiv 2021.11.29.470343

Backbone

• Vector backbone: pFastBac Dual
• Backbone manufacturer: Thermo Fisher (Invitrogen)
• Backbone size w/o insert (bp): 5238
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ATP1A1
• Species: R. norvegicus (rat)
• Insert Size (bp): 3072
• GenBank ID: NM_012504.1
• Entrez Gene: Atp1a1 (a.k.a. Nkaa1b)
• Promoter: PH

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATTCCGGATTATTCATACCGTCCCACCATCG
• 3′ sequencing primer: GTGGTATGGCTGATTATGATCCTCTAGTACTTCTC

Gene/Insert 2

• Gene/Insert name: ATP1B1
• Species: R. norvegicus (rat)
• Insert Size (bp): 915
• GenBank ID: NM_013113.2
• Entrez Gene: Atp1b1 (a.k.a. ATPBS)
• Promoter: p10

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: PaeI (unknown if destroyed)
• 5′ sequencing primer: CGGGTTCCTTCCGGTATTGTCTCCTTC
• 3′ sequencing primer: ACGGACCTTTAATTCAACCCAACACAATATA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.11.29.470343v5 for bioRxiv preprint.