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Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/CD63mScarlet
Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorInsertCD63-mScarlet (CD63 Human)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xHA-hTERT
Plasmid#51637PurposeMammalian expression of 3xHA tagged human TERT, N-terminal.DepositorInsertTERT (TERT Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-mitoDsRed
Plasmid#44386Purpose3rd generation lentiviral vectorDepositorInsertmitoDsRed
UseLentiviralTags61 aa targeting sequence of the P1 isoform F1F0-A…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CD9-10
Plasmid#55013PurposeLocalization: Tetraspanin/Membrane, Excitation: 587, Emission: 610DepositorInsertCD9 (CD9 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003
Plasmid#133447PurposeExpresses SpyCatcher003 in bacterial cytoplasmDepositorInsertSpyCatcher003
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmal_c5x_RNAse_Inhib
Plasmid#153314PurposeBacterial expression of an RNase inhibitorDepositorInsertRNAse Inhibitor
UseTagsMaltose Binding ProteinExpressionBacterialMutationPromoterAvailable sinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MaLionG
Plasmid#113906PurposeGreen ATP sensorDepositorInsertMaLionG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
HDAC4 Flag
Plasmid#13821PurposeMammalian expression of human histone deacetylase 4 with flag tagDepositorInsertHDAC4 (HDAC4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABE GFP
Plasmid#10668PurposeEmpty backbone for retroviral gene expression. Select recipient cells using GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
Plasmid#179105PurposepCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINTDepositorInsertpCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE
Plasmid#180280PurposeThe CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.DepositorInsertCasMINI sgRNA (backbone) and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA
Plasmid#158990PurposeVector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cellsDepositorInsertdSaCas9-VP64
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Fibrillarin
Plasmid#26673DepositorInsertFibrillarin (FBL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAluYa5-neo-TET
Plasmid#51283Purposeexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertAluYa5
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterupstream pol III enhancer sequence of the 7SL gen…Available sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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TagBFP2-CAAX
Plasmid#116856Purposeplasma membrane markerDepositorInsertHRAS
UseTagsTagBFP2ExpressionMammalianMutationAmino acids 172-189PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)
Plasmid#169188PurposeTo express His-SUMO-nsp12 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_ORI vector
Plasmid#99296PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only