Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

160,747 results

Showing: 1921 - 1940 of 160747 results

  1. pETDuet-TFIIE

    Plasmid
    #171083
    Purpose
    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171083-plasmid-map-sequence-id-335640

    Enlarge

  4. pLentiDP1.0

    Plasmid
    #171094
    Purpose
    lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2
    Depositor
    Dario Palmieri
    Article
    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1
    Insert
    OsTIR1-mEmerald-P2A-miniAID-mCherry2
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    171094-plasmid-map-sequence-id-339473

    Enlarge

  5. pLentiDP1.3

    Plasmid
    #171096
    Purpose
    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2
    Depositor
    Dario Palmieri
    Article
    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1
    Insert
    OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
    Use
    Lentiviral and Synthetic Biology
    Tags
    Emerald, cdt1, miniAID, mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171096-plasmid-map-sequence-id-339099

    Enlarge

  6. pST50Trc1-HISNDHFR

    Plasmid
    #63940
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63940-plasmid-map-sequence-id-177346

    Enlarge

  7. HypaCas9

    Plasmid
    #138557
    Purpose
    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    HypaCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138557-plasmid-map-sequence-id-274767

    Enlarge

  9. pMSCVpuroATT

    Plasmid
    #47539
    Purpose
    Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
    Depositor
    David Weinstock
    Article
    Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pLentiDP1.2

    Plasmid
    #171095
    Purpose
    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2
    Depositor
    Dario Palmieri
    Article
    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1
    Insert
    OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
    Use
    Lentiviral and Synthetic Biology
    Tags
    Emerald, Geminin, miniAID, mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    171095-plasmid-map-sequence-id-339475

    Enlarge

  11. TFORF3194

    Plasmid
    #144670
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TLX3 (TLX3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144670-plasmid-map-sequence-id-350513

    Enlarge

  13. Donor_eGP2AP_RC

    Plasmid
    #133784
    Purpose
    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.
    Depositor
    Sriram Kosuri
    Article
    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d
    Type
    Empty backbone
    Use
    Tags
    eGFP-P2A-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133784-plasmid-map-sequence-id-274492

    Enlarge

  16. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid
    #197499
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197499-plasmid-map-sequence-id-386708

    Enlarge

  19. pST50Trc3-HISNDHFR

    Plasmid
    #63976
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63976-plasmid-map-sequence-id-177382

    Enlarge

  20. pST50Trc4-HISNDHFR

    Plasmid
    #63994
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63994-plasmid-map-sequence-id-177400

    Enlarge

Showing: 1921 - 1940 of 160747 results