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H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
UseTagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone
Plasmid#164266PurposesgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6 promoter from W05B2.8Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG5-mERRbeta-short
Plasmid#52188PurposeExpresses mouse ERRbeta short form splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-MKL1 D301-380
Plasmid#19852DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 301 to 380PromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
p5E-pax3a-5.4k
Plasmid#164966Purposezebrafish pax3a -5.4k promoterDepositorInsertpax3a (pax3a Zebrafish)
UseGateway 5 end entry vectorTagsExpressionMutationpromoter -5.4kPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPTXLUC
Plasmid#68504Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutationPromoterOPTXAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 T450A
Plasmid#19843DepositorInsertMKL1 T450A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Threonine 450 to AlaninePromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn1b
Plasmid#164957PurposeZebrafish kcnn1b gene CDSDepositorInsertkcnn1b (kcnn1b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-MKL1 D381-506
Plasmid#19853DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 381 to 506PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG-NSarQ27G-VMAn-FKBP
Plasmid#70229PurposeExpresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicingDepositorInsertalpha Sarcin (N-terminal portion)
UseTags3XFLAG, FK506- and rapamycin-binding protein (FKB…ExpressionMammalianMutationChanged Gln 27 to GlyPromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 L272A
Plasmid#19850DepositorInsertMKL1 L272A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 272 to AlaninePromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S449A
Plasmid#19842DepositorInsertMKL1 S449A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 449 to AlaninePromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-SMN2-inclusion-GLuc
Plasmid#218671PurposeAlternative splicing signaling: signal increased with Ex7 inclusion increasingDepositorInsertSMN2 (SMN2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_AAVS1-OsTIR1-F74G-SNAP
Plasmid#217665PurposeRepair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locusDepositorInsertOsTir1 F74G-SNAP-Blast-AAVS1
UseTagsSNAPExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-SFFV_NKX2-1_Short_V5-GFP
Plasmid#221500PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_CdTag-HA
Plasmid#221493PurposeNKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_DelCTerm_1-223AA
Plasmid#221476PurposeGateway cloning of NKX2-1 C-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 224-371AA C-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only
