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Plasmid#164803Purposemammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLSDepositorInsertRecT
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UAS-CITRINE
Plasmid#104839PurposeSPARK reporter geneDepositorInsertCitrine
UseAAVTagsExpressionMammalianMutationPromoterUASAvailable sinceJan. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Lyn11-FRB
Plasmid#155228Purposemembrane anchor for FKBP-FRB systemDepositorInsertMembrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x2
Plasmid#58772PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9-MS2-BB_BbsI
Plasmid#164802PurposeREDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKvenus-Scribble
Plasmid#58738PurposeExpresses Venus-tagged human Scribble proteinDepositorInsertScribble (SCRIB Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
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HA-FOXO3a TM
Plasmid#1788DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationT32A, S253A, S315A (no longer detectably phosphor…PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPPAT-EGFP
Plasmid#99105PurposeExpresses human PPAT-EGFP fusion in mammalian cellsDepositorInsertPPAT (PPAT Human)
UseTagsEGFP (enhanced green fluorescent protein)ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-SOD2-2A-Catalase-WPRE
Plasmid#67635PurposeAAV vector expressing both SOD2 and mitochondrial targeted CatalaseDepositorUseAAVTagsExpressionMutationDeleted the Peroxisome Targeting Signal from Cata…PromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
CoV2-M-IRES-E
Plasmid#177938PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Mef2c-tPT2A-GFP
Plasmid#111771PurposeBicistronic construct: Co-express two genes by 2A peptidesDepositorInsertMef2c-tPT2A-GFP (Mef2c Mouse)
UseCloning intermediateTagsGFPExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flp-DOG
Plasmid#80084PurposeFlp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cellsDepositorInsertFlp-DOG
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_synNotch_TetRVP64
Plasmid#79126PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_TetRVP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA
Plasmid#116771PurposeLentiviral expression of PIK3CADepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox2
Plasmid#213726PurposeMammalian cell expression vector for overexpression of Rbfox2 in motor neurons.DepositorInsertRbfox2 (Rbfox2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCgCas9_recT
Plasmid#153337PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; KanrDepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180266PurposeLentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNADepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
3X FLAG-FUS-WT
Plasmid#44985DepositorInsertFUS (Full length) (FUS Human)
UseTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSUMO1
Plasmid#52258PurposeBacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA(CTRL)-CMV-eGFP
Plasmid#194017PurposeExpresses a gRNA that targets the LacZ gene (serves as control) and eGFPDepositorInsertssgRNA(CTRL)
eGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Cas9-2A-GFP-sgRNA
Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPExpressionMutationPromoterMSCVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Lats1 (Nigg HS189)
Plasmid#41156DepositorInsertLats1 (LATS1 Human)
UseTags3xMyc and PreScission SiteExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9n-MS2-BB_BbsI
Plasmid#164804PurposeREDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-Wt-NmeCas9
Plasmid#71474Purposebacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-WT-NmeCas9
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-NE2h-IRES-mCherry-CAAX
Plasmid#208691PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)
Plasmid#48308DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-R-Creon shRNA[Control]
Plasmid#180775PurposeThis vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.DepositorTypeEmpty backboneUseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NLyn-Venus-Zdk1
Plasmid#81042Purposemammalian expression of plasma-membrane anchored Zdk1DepositorInsertZdk1
UseTags6x His tag, FLAG, NLyn, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
mcherry-DN KASH
Plasmid#125553Purposemcherry labeled DN-KASHDepositorInsertSYNE1 (SYNE1 Human)
UseTagsmcherryExpressionMammalianMutationTruncated form: only the KASH domain of nesprin-…PromoterCMVAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC1A5_STOP
Plasmid#161140PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC1A5 (SLC1A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti_dCas9-2xAM
Plasmid#92220PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sitesDepositorInsertsSp-dCas9-2xAM tag
gRNA to be inserted into Bbs I sites
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6Available sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 YFP
Plasmid#84911Purposeexpression of TDP-43 YFP in mammalian cellsDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIG-641 JE-TRAC-BCMA(BB)28z-P2A
Plasmid#186127PurposeBCMA-CAR DNA productionDepositorInsertBCMA-CAR (TNFRSF17 Human)
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bsd
Plasmid#214370PurposeCloning vector with BlasticidinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-CD19 puro
Plasmid#196634PurposeExpress CD19DepositorInsertCD19 (CD19 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3HA-kanMX6
Plasmid#39295DepositorInsertKanR
UseYeast genomic targetingTags3HA, A. gossypii translation elongation factor 1a…ExpressionMutationPromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 N C-V5-tag
Plasmid#179987PurposeMammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 N
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 N C-V5-tag
Plasmid#179968PurposeMammalian expression vector for SARS-CoV-1 N, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 N (N Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
enAsABE8e
Plasmid#138506PurposeA-to-G base editorDepositorInsertecTadA(8e)-dAsCas12a (En)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 C-V5-tag ORF3a
Plasmid#179969PurposeMammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 ORF3a (ORF3a Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-CAG-CyRFP1
Plasmid#84357PurposeExpresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)DepositorInsertCyRFP1
UseAAVTagsExpressionMammalianMutationDouble FLEXedPromoterCAGAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 ORF3a C-V5-tag
Plasmid#179988PurposeMammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 ORF3a
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF2528
Plasmid#144127PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertERCC6 (ERCC6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->HA-mtLaG2
Plasmid#162225PurposeLentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cellsDepositorInsertanti-GFP nanobody LaG2
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only