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Plasmid#155237Purposefor mammalian overexpression of the mutated ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19CSdomains
Plasmid#155240Purposefor mammalian overexpression of the CS domains of rat USP19DepositorInsertUSP19 (CS domains) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ shRNA-2
Plasmid#160068PurposeKnock Down SH3+ Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ 3m shRNA-1
Plasmid#160067Purpose3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGGcTGaTTaCAACAAGGATTG (mutations shown in lower c…Promotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ shRNA-1
Plasmid#160066PurposeKnock Down SH3+ Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA3
Plasmid#160213PurposeKnock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationTGTATGACCTCaGGcTGtACCA (mutations shown in lower …Promotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA2
Plasmid#160210PurposeKnock Down MTLSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA3
Plasmid#160212PurposeKnock Down MYDSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA2
Plasmid#160211PurposeKnock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationTGACGTTGCTCaGGcTGtACCA (mutations shown in lower …Promotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA1
Plasmid#160209PurposeKnock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…Promotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA1
Plasmid#160208PurposeKnock Down MQWSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine
Plasmid#160069PurposeConditionally overexpress collybistin (CB2SH3-) and mCitrineDepositorInsertARHGEF9 (Arhgef9 Rat)
UseAAVTagsHA- in CB2SH3ExpressionMutationPromoterhuman Synapsin promoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 APOBEC3 no UGI
Plasmid#135350PurposeExpression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.DepositorInsertnSpyCas9-rAPOBEC3-ID-1058
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP APOBEC3 no UGI
Plasmid#135352PurposeExpression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminusDepositorInsertrAPOBEC3-nSpyCas9 aa 1029
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 AIDx with UGI
Plasmid#135354PurposeExpression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminusDepositorInserthAIDx-nSpyCas9 aa-UGI
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER-CRmut
Plasmid#155253Purposefor mammalian overexpression of the mutated ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) CR mutant (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationCys(545) to ArgPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19CYT-CAmut
Plasmid#155250Purposefor mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
UseTagsExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19ER-CAmut
Plasmid#155249Purposefor mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (ER isoform) CA mutant (Usp19 Rat)
UseTagsExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19CYT
Plasmid#155248Purposefor mammalian overexpression of the CYT isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (Cytoplasmic isoform) (Usp19 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19ER
Plasmid#155247Purposefor mammalian overexpression of the ER isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only