Addgene

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pNAHA

Plasmid

#44169

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

hemagglutinin gene
neuraminidase gene

Use

Tags

Expression

Mammalian

Mutation

T55A

Promoter

CMV enhancer/human Ferritin light chain promoter …

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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PBCAG-eGFP

Plasmid

#40973

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Inserts

PiggyBac transposon 3' terminal repeats
PiggyBac 5' terminal repeats

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP hsp70

Plasmid

#15215

Depositor

Lois Greene

Article

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 (HSPA1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-neo (1795)

Plasmid

#41000

Depositor

Jonathon Pines

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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MP1

Plasmid

#69627

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 umuD' umuC recA730

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-VP3SA11

Plasmid

#89164

Purpose

Express Rotavirus SA11 VP3 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA blast

Plasmid

#104993

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLGB13

Plasmid

#126618

Purpose

Suicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-pCMV-PfV-GS-Sapphire

Plasmid

#116933

Purpose

Mammalian vector for expression of the 40nm GEM

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Nsp3 -EGFP

Plasmid

#165108

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-PTE2A

Plasmid

#111773

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA puro

Plasmid

#104990

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/38.1R]

Plasmid

#114492

Purpose

Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 HA C9ORF72 op

Plasmid

#74156

Purpose

Express HA tagged optimized C9ORF72 in mammalian cells

Depositor

Nicolas Charlet-Berguerand

Insert

C9ORF72 (C9orf72 Synthetic, Human)

Use

Tags

HA

Expression

Mammalian

Mutation

optimized sequence

Promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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His6-MBP-uTEV3

Plasmid

#135464

Purpose

Bacterial expression of uTEV3 (full-length) protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

His6-MBP-uTEV3

Use

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation (improves stability) , I138T/S153N…

Promoter

T5

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-tdTomato

Plasmid

#28306

Depositor

Edward Boyden

Has Service

AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

tdTomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Atg12

Plasmid

#24923

Depositor

Toren Finkel

Article

Lee et al J Biol Chem. 2009 Mar 6. 284(10):6322-8.

Insert

Atg12 (ATG12 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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