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Plasmid#45877DepositorInsertParkin (PRKN Human)
UseTagsEGFPExpressionMammalianMutationC431S mutation (ubiquitin acceptor site)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR (in lacZ deficient strain)
Plasmid#99245PurposeFor autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17
Plasmid#193298Purposeepisomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCsDepositorUseTagsExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNA
Plasmid#215560PurposeConstitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanRDepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DogCatcher
Plasmid#171772PurposeExpresses DogCatcher protein in bacterial cytoplasmDepositorInsertDogCatcher
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
Plasmid#117484PurposeExpresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cellsDepositorInsertSpyCatcher002-6aa-sfCherry2(1-10)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR4
Plasmid#116743PurposeLentiviral expression of FGFR4DepositorInsertFGFR4 (FGFR4 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Atl3
Plasmid#86680PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorInsertATL3 (ATL3 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only