Skip to main content
Addgene
Showing: 9491 - 9500 of 153597 results

  1. pAD-LyseR (in lacZ deficient strain)

    Plasmid
    #99245
    Purpose
    For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    phage lambda gene R (R phage lambda)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    beta-lactamase promoter
    Available since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99245-plasmid-map-sequence-id-191039

    Enlarge

  4. pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

    Plasmid
    #193298
    Purpose
    episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    193298-plasmid-map-sequence-id-379909

    Enlarge

  6. G-CaMP3

    Plasmid
    #22692
    Purpose
    a single-wavelength GCaMP2-based genetically encoded calcium indicator
    Depositor
    Loren Looger
    Article
    Tian et al Nat Methods. 2009 Dec;6(12):875-81.
    Insert
    G-CaMP3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N363D mutation (increased fluorescence)
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    22692-plasmid-map-sequence-id-241043

    Enlarge

  7. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Article
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    85452-plasmid-map-sequence-id-173969

    Enlarge

Showing: 9491 - 9500 of 153597 results