Addgene

Showing: 1881 - 1900 of 35240 results

Results per page:

Sort results by:

pCDNA3 Flag DLC1

Plasmid

#24265

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-hIFITM3

Plasmid

#58461

Purpose

Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
Ii-Str_ManII-SBP-EGFP

Plasmid

#65255

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Use

Tags

flag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPV25.1_dGFPmut3-AAV

Plasmid

#187377

Purpose

rpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

Depositor

Roi Avraham

Article

Ciolli Mattioli et al Curr Biol. 2023 Nov 20;33(22):4880-4892.

Insert

dGFPmut3_AAV

Use

Tags

AAV

Expression

Bacterial

Mutation

C-terminal AAV tail

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-scFvGCN4sfGFP-VP64-GB1

Plasmid

#141417

Purpose

Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

Depositor

Izuho Hatada

Article

Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

Insert

scFvGCN4sfGFP-VP64-GB1

Use

Tags

HA and sfGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GRK2-CAAX

Plasmid

#166224

Purpose

GRK2-CAAX mammalian expression plasmid

Depositor

Robert Lefkowitz

Article

Inglese et al Nature. 1992 Sep 10;359(6391):147-50. do

Insert

bovine GRK2-CAAX (GRK2 Bovine)

Use

Tags

no

Expression

Mammalian

Mutation

adding CAAX sequence at C terminal

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV4-p100

Plasmid

#23287

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

NFkB p100 subunit (NFKB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pRham-CALM1

Plasmid

#182500

Purpose

Bacterial expression of the Homo sapiens calmodulin, CALM1

Depositor

Dan Mulvihill

Article

Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

Insert

CALM1 (CALM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP FAT

Plasmid

#50517

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase, domain

Use

Tags

GFP

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

Plasmid

#172225

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK364.306

Plasmid

#192642

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM60-Auf1

Plasmid

#38242

Purpose

3rd generation lentiviral vector

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

AUF1 (HNRNPD Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabepuro-HA-GASC1

Plasmid

#24182

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pBluescript-mouse cyclin D2

Plasmid

#86791

Purpose

bacterial expression of mouse cyclin D2

Depositor

Charles Sherr, Martine Roussel

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D2 (Ccnd2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70Tet(DadR)-DadR

Plasmid

#191636

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLEX+mCyclin D1

Plasmid

#86793

Purpose

mammalian expression of cyclin D1

Depositor

Charles Sherr, Martine Roussel

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D1 (Ccnd1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
hRIP1 HA GFP K45A

Plasmid

#41389

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP1 (RIPK1 Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

Kinase Domain Mutant (K45A)

Promoter

CMV

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pGL2-Basic-Myogenin core promoter

Plasmid

#134722

Purpose

Myogenin core promoter in a luciferase reporter plasmid

Depositor

Michael Chin

Article

Sun et al J Biol Chem. 2001 May 25;276(21):18591-6

Insert

Myogenin Promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Myogenin

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-MAGE-2-429

Plasmid

#89090

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only