-
Plasmid#119299PurposeExpresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logicDepositorInsertsplit nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
UseLuciferaseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
UseTagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8 Flag2AUF1(p40)
Plasmid#135945PurposeExpresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.DepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2ExpressionMammalianMutationPromoterCMV and SP6Available sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8 Flag2AUF1(p42)
Plasmid#135946PurposeExpresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.DepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2ExpressionMammalianMutationPromoterCMV and SP6Available sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
AP4mS_PPVs_P3_cLuc
Plasmid#119300PurposeExpresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logicDepositorInsertsplit cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F3shRNA human
Plasmid#66885PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F3DepositorInsertE2F transcription factor 3 (E2F3 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-NFYC-IDR-mEGFP
Plasmid#185015PurposeBacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion ProteinDepositorInsertNFYC IDR (Nfyc Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-N-gamma-tubulin (1-333)
Plasmid#87858PurposeFluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationFragment from aa 1 to 333PromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
HEL binding scFv D44.1
Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1
Plasmid#111859PurposeFull length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, TEV site, and ZZExpressionInsectMutationPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-puro/hFOXH1 shRNA1
Plasmid#59303PurposeKnockdown of human FOXH1DepositorInserthFOXH1 shRNA (FOXH1 Human)
UseRNAi and RetroviralTagsExpressionMutationPromoterhU6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hFOXH1GR
Plasmid#59311PurposeForced expression of human FOXH1GRDepositorInsertFOXH1 (FOXH1 Human)
UseRetroviralTagscGR - hormone binding domain of glucocorticoid re…ExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-CRY2PHR-SID4X
Plasmid#63663PurposeAAV expression of CRYS2PHR-SID4XDepositorInsertSID4X-CYR2PHR
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-puro/hFOXH1 shRNA3
Plasmid#59308PurposeKnockdown of human FOXH1DepositorInserthFOXH1 shRNA (FOXH1 Human)
UseRNAi and RetroviralTagsExpressionMutationPromoterhU6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad3
Plasmid#89828PurposeKnockdown human Smad3 expression in human mammalian cellsDepositorInsertSMAD3 shRNA (SMAD3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CMV_oROS-G_LF(C199S)
Plasmid#216112PurposeExpresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.DepositorInsertoROS-G_LF(C199S)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-G_LF(C199S)
Plasmid#216116PurposeEncodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.DepositorInsertoROS-G_LF(C199S)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse+R111Q–insD
Plasmid#191229PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved aspartic acid at 121 and replaced arginin…PromoterPH and p10Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMETn-del-gRNA2
Plasmid#185007Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMETn-del-gRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-IAP-del-gRNA1
Plasmid#185002Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertIAP-del-gRNA1 (Akp3 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only