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GB4636

Plasmid

#215264

Purpose

Module for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

Etr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes 1-480 WT

Plasmid

#158541

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Luciferase

Plasmid

#213979

Purpose

Expresses Doxy Inducible Luciferase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Luciferase

Use

Lentiviral

Tags

6xHis affinity tag

Expression

Bacterial and Mammalian

Mutation

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5A tail only

Plasmid

#166956

Purpose

Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A (820-1027aa)

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

Plasmid

#158542

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion, F188D, W2…

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC-S227A

Plasmid

#200560

Purpose

Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

CMV promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1B-WT-Flag-UTR

Plasmid

#183239

Purpose

Expression of FLAG tagged HNF1B

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox B (HNF1B Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CuO-V5 CASC3 siRes 110-480 WT

Plasmid

#158543

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 110-480, C-terminal deletion

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5A delta Tail

Plasmid

#166955

Purpose

Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A (1-820aa)

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN

Plasmid

#209786

Purpose

Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

TNT4, TadA8e, SpRY N, inteinN

Use

AAV

Tags

Expression

Mutation

Promoter

TNT4

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1B-FUS-IDR

Plasmid

#183240

Purpose

Expression of HNF1B fused to a intrinsically disordered regions of the FUS protein

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox B (HNF1B Human)

Use

Tags

Fusion to an IDR from the FUS protein and FLAG ta…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1A-delta-IDR2

Plasmid

#183238

Purpose

Expression of FLAG tagged HNF1A with deletion of a intrinsically disordered regions (AA545-573)

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Deletion of amino acids 545-573

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187287

Purpose

Express pmCherry-IL2-AH-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-2A-RhoA Q63L

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1

Plasmid

#187292

Purpose

Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2, RhoA Q63L

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-KLC1 delta Tail

Plasmid

#166965

Purpose

Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

KLC1 (1-495aa)

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5AR280H

Plasmid

#166957

Purpose

Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A-R280H (Kif5a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A-R280H

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CuO-V5-CASC3 siRes 110-480 188/218-mut

Plasmid

#158544

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 110-480, C-terminal deletion, F188D, …

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7-E383K

Plasmid

#113731

Purpose

Express mutant RGS7- E383K- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

E383K

Promoter

CMV

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7-R44C+V56C

Plasmid

#113730

Purpose

Express double mutant RGS7- R44C+V65C in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R44C and V56C

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only
pCDF1-MCS2-EF1-Puro-RGS7-R44C

Plasmid

#113729

Purpose

Express mutant RGS7- R44C- in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge