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Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorInsertGα11-LgB97 (GNA11 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα13-LgB106
Plasmid#134364PurposeNanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.DepositorInsertGα13-LgB106 (GNA13 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-FLucStop
Plasmid#177617PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationIn-frame stop codon added between FLuc and FSEPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-Gluc-RMA-IRES-EGFP
Plasmid#189628PurposeExpresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianMutationPromoterIRES and TREAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-DIO-intron336-dLUC
Plasmid#110056PurposeCry1 transcription luciferase reporterDepositorInsertCry1 promoter and luciferase (Cry1 Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCry1Available sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3 2 kb prom. and exon 1 fragment CD274
Plasmid#107007PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM19
Plasmid#123655PurposeEncodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cellsDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionInsectMutationPromoterpIExAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1
Plasmid#123194Purposebinary plant vector for transient dual luciferase (rLUC & fLUC with introns) expressionDepositorInsertpNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-FL
Plasmid#202515PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGL4-4X Mut Renilla
Plasmid#70040PurposeControl plasmid for testing 5'UTR folding elementsDepositorInsertRandom sequence matched for GC-content
UseLuciferaseTagsrenilla luciferaseExpressionMammalianMutationPromoterSV40Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3 1 kb prom and exon1 fragment CD274
Plasmid#107006PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-321/+120
Plasmid#35540DepositorUseLuciferaseTagsluciferaseExpressionMutationPromoternoneAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-1574/+120
Plasmid#35539DepositorUseLuciferaseTagsluciferaseExpressionMutationPromoternoneAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-LPPRC-mod
Plasmid#48168Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame elementDepositorInsertLRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
UseLuciferaseTagsExpressionMutationA/T mutation in the ATG of the uORFPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMCP-MU-Luc (MCP-1 mut promoter in pGL3-basic)
Plasmid#40325DepositorInsertMCP-1 promoter (mutated) (Ccl2 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationcontains MCP-1 promoter region through the nucleo…PromoterMCP-1Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMiR-RECK-3'UTR-miR-21-mut
Plasmid#53693PurposeLuciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding siteDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseTagsExpressionMutationnucleotide sequence 1083-1086 is mutated to TTCGPromoterAvailable sinceJuly 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_01
Plasmid#158102Purpose5'UTR stem-loop luciferase reporter: 0kcal/molDepositorInsert5'UTR synthetic stem-loop 0kcal/mol
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-SV40P-Tet2 enhancer H1
Plasmid#63883PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet2DepositorInsertTet2 enhancer fragment H1
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNeuLite PEA3 mt
Plasmid#16248DepositorInsertHer-2/neu promoter (ERBB2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPEA3 mt: PEA3 motif AGGAAG changed to AGCTCGPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-Lin28b-P3
Plasmid#64794PurposeLuciferase expression driven by Lin28b promoter P3DepositorInsertLin28b promoter P3 (LIN28B Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only