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Plasmid#47734PurposeExpresses enzymatically monobiotinylated full-length MSP11 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP11
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSRP1-bio
Plasmid#47736PurposeExpresses enzymatically monobiotinylated full-length MSRP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSRP1
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSRP2-bio
Plasmid#47737PurposeExpresses enzymatically monobiotinylated full-length MSRP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSRP2
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSRP3-bio
Plasmid#47738PurposeExpresses enzymatically monobiotinylated full-length MSRP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSRP3
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
AARP-bio
Plasmid#47783PurposeExpresses enzymatically monobiotinylated full-length AARP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised AARP
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pf34-bio
Plasmid#47784PurposeExpresses enzymatically monobiotinylated full-length Pf34 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised Pf34
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP4-bio
Plasmid#47717PurposeExpresses enzymatically monobiotinylated full-length MSP4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP4
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG225 HIPK1 3'UTR mut
Plasmid#12044DepositorInsertHIPK1 3'UTR mut (HIPK1 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R53E/R59E/R61E
Plasmid#60355PurposeR53E R59E R61E triple mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR53E, R59E and R61E mutations; contains amino aci…PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG73 PFTK1 3'UTR mut
Plasmid#12061DepositorInsertPFTK1 3'UTR mut (CDK14 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta N54
Plasmid#40934DepositorInsertEPLIN (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 1-54PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta N112
Plasmid#40935DepositorInsertEPLIN (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 1-112PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta N173
Plasmid#40936DepositorInsertEPLIN (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 1-173PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG177 HIPK1 3'UTR mut
Plasmid#12035DepositorInsertN10 3'UTR mut (HIPK1 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG221 MDFIC 3'UTR mut
Plasmid#12051DepositorInsertN22 3'UTR mut (MDFIC Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28Man
Plasmid#203399Purposeexo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymesDepositorInsertman2A
UseTagsH6ExpressionBacterialMutationNonePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_NIPBL_mEGFP_mAID
Plasmid#217664PurposeRepair template to endogenously tag NIPBL with mEGFP-mAID TagDepositorInsertNIPBL_mEGFP_mAID (NIPBL Human)
UseTagsmAID and meGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Cas9-hGem_gRNA2
Plasmid#217659Purposeexpresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locusDepositorInsertsgRNA2 targeting the human AAVS1 safe harbour locus
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI2B-3×FLAG
Plasmid#215510PurposeExpresses FLAG tagged human WIPI2B.DepositorInsertWD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)
UseRetroviralTags3×FLAGExpressionMammalianMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-WT
Plasmid#210722PurposeConstruct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only