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Plasmid#138328Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1b
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
APEX2-V5-G3BP1_pTRE-G418
Plasmid#202013Purposeexpresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertAPEX2-V5-G3BP1
UseLentiviralTagsExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAT005
Plasmid#171632Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-B2M
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab11 WT
Plasmid#12674DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-HRP-TM_pLX304
Plasmid#202021Purposeexpresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vectorDepositorInsertFlag-GFP-HRP-TM
UseLentiviralTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3A-BE5-NG-BlastR
Plasmid#152998PurposeHigh purity C-to-T base editorDepositorInserteA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-141
Plasmid#118557PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-Ef1a-CreN-InteinN
Plasmid#187614PurposeAAV vector that encodes N-terminal portion of split-Intein-mediated split-CreDepositorInsertCreN-InteinN
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-Ef1a-InteinC-CreC
Plasmid#187615PurposeAAV vector encoding C-terminal portion of split-Intein-mediated split-CreDepositorInsertInteinC-CreC
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-143
Plasmid#118559PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 T204D
Plasmid#80877Purposemammalian expression of constitutively active ALK5 T204DDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationT204D (constitutively active)PromoterCMVAvailable sinceAug. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC
Plasmid#126218PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIaAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
myc-hAtg13
Plasmid#31965DepositorInsertAtg13 (ATG13 Human)
UseTagsmycExpressionMammalianMutationPromoterCMV, SP6Available sinceSept. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-EA-BE4max-NG-BlastR
Plasmid#152992PurposeHigh purity C-to-T base editorDepositorInsertBE4 H47E+S48A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAT003
Plasmid#171628Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-TRAC
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and EF1-aAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-HIS-CBM-Xmod-DocIII
Plasmid#91698PurposeCBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGGDepositorInsertGGG-HIS-CBM-Xmod-DocIII
UseTagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-LPETGG
Plasmid#91697PurposeCohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGGDepositorInsertCohIII-CBM-HIS-LPETGG
UseTagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK3082
Plasmid#78742PurposeHuman expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertLbCpf1 crRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(40nm)-LPETGG
Plasmid#90475PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(40nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-STX17TMΔC
Plasmid#221737PurposeStable expression of GFP-STX17TMΔC in mammalian cellsDepositorInsertSTX17 (STX17 Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-228 and 227-302PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TB:5xNF- κβ_RE:MiniP
Plasmid#133878Purpose5 copies of the NF-κβ DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
5xNF-kb_RE::RedF
Plasmid#124530PurposeNFKB RedFirefly luciferase reporterDepositorInsertNF-κβ RedF reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
Plasmid#46168DepositorInsertcodon optimized Cas9_SV40 NLS with intron
UseCRISPRTagsExpressionMutationintron inserted at position 3113-3163, and SV40 N…Promotereef-1A.1 (eft-3)Available sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGS1021
Plasmid#59575PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagExpressionMutationPromoterNoneAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre
Plasmid#203838PurposeFlp-dependent Cre and mScarlet-KASH expressionDepositorInsertCre
UseAAVTagsmScarlet-KASH-2AExpressionMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-132
Plasmid#118518PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab11 WT
Plasmid#12679DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92392Purposeproduces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTADepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHybrid-Electra2
Plasmid#179475PurposeExpresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cellsDepositorInsertsElectra2
mScarlet-I
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a)
Plasmid#79672PurposeCre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP (Scn9a Mouse, jellyfish)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
LBR-mCherry
Plasmid#172446PurposeExpression of nuclear envelope protein LBR with a C-terminal mCherry tagDepositorInsertlamin B receptor (LBR Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
35S-GWY-GAL4BD/pAM
Plasmid#201231PurposeUsed to produce and express in planta N-terminal fusion proteins with the GAL4 BDDepositorInsert35S-GWY-GAL4BD
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-RhoBAST-MS2
Plasmid#196342PurposeOverexpression of circular RhoBAST-MS2DepositorInsertTornado-RhoBAST-MS2
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST PKAKTRClover
Plasmid#59153PurposeLentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertPKA (PRKACA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA human NEMO
Plasmid#13512DepositorInsertNEMO (IKBKG Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-FOS
Plasmid#33353DepositorInsertFOS (Fos Mouse)
UseTagsFLAGExpressionMammalianMutationDominant Negative of c-Jun; heterodimerizes and i…PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUbC-OsTIR1-myc-IRES-scFv-sfGFP
Plasmid#84563PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFPDepositorInsertTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
UseLentiviralTagsmyc tag (9x)ExpressionMutationPromoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
MI-Luciferase-IRES-mCherry
Plasmid#75020PurposeEncodes the expression of Luciferase and mCherryDepositorInsertsfirefly Luciferase
mCherry
UseLuciferase and RetroviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mcherry-Myo 9b
Plasmid#134918PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.DepositorInsertMyo 9b (rat) with 3' UTR (Myo9b Rat)
UseTagsmcherryExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertlacI (lacI Escherichia coli)
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-Electra2
Plasmid#184926PurposeExpresses paxillin fused to blue fluorescent protein Electra2DepositorInsertpaxillin-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP3
Plasmid#42310DepositorInsertVAMP 3 (VAMP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1
Plasmid#17743DepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVDL9.3
Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LYN11-FRB-CFP
Plasmid#38003DepositorUseTagsECFPExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only