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Showing: 3761 - 3800 of 149038 results

  1. BPK1520_blastR

    Plasmid
    #175289
    Purpose
    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection
    Depositor
    Ronald Cohn
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    175289-plasmid-map-sequence-id-346344

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  8. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #179682
    Purpose
    Expression of base editor in mammalian cells and for cell sorting
    Depositor
    David Liu
    Article
    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387
    Insert
    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S1330I A1341V N1342S E1370K T1380I
    Promoter
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179682-plasmid-map-sequence-id-355098

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  9. pET-16b-10His-TEV-MCES_VACCA_D1_D12

    Plasmid
    #203671
    Purpose
    The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage
    Depositor
    Stephen Cusack
    Article
    Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:
    Insert
    MCES_VACCA_D1_D12
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    203671-plasmid-map-sequence-id-403167

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  10. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

    Plasmid
    #214138
    Purpose
    Insect cell expression of human 20S CP alpha-subunits
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    PSMA5 (PSMA5 Human)
    PSMA6 (PSMA6 Human)
    PSMA7 (PSMA7 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214138-plasmid-map-sequence-id-431242

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  13. pLXRN-EGFP

    Plasmid
    #99207
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Peter Pedersen
    Insert
    EGFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    A Kozak sequence was incorporated to enhance tran…
    Promoter
    LTR
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99207-plasmid-map-sequence-id-190957

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  19. pSAB35

    Plasmid
    #175787
    Purpose
    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag
    Depositor
    John Rubinstein
    Article
    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246
    Insert
    SidK (1-278)
    Use
    Tags
    3xFLAG and 6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    175787-plasmid-map-sequence-id-348166

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  28. GSN-bio-His

    Plasmid
    #52067
    Purpose
    Expresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    GSN (GSN Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52067-plasmid-map-sequence-id-80292

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  29. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162075-plasmid-map-sequence-id-325304

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  30. TFORF3286

    Plasmid
    #144762
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR2F6 (NR2F6 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144762-plasmid-map-sequence-id-404377

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  31. CACNA2D1-TwinStrepII

    Plasmid
    #83192
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CACNA2D1 (Cacna2d1 Rat)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83192-plasmid-map-sequence-id-163487

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  32. pOpen-T4gene43

    Plasmid
    #165524
    Purpose
    Polymerase that replicates viral genomic DNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165524-plasmid-map-sequence-id-338922

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  34. pRRLSIN.cPPT.PGK-GFP.WPRE

    Plasmid
    #12252
    Purpose
    3rd generation lentiviral backbone
    Depositor
    Didier Trono
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2007
    Availability
    Academic Institutions and Nonprofits only
    12252-plasmid-map-sequence-id-444115

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Showing: 3761 - 3800 of 149038 results