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pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS8-HaloTagx2
Plasmid#177113PurposeExpresses Vps8-HaloTagx2 in yeast cellsDepositorInsertVPS8 (VPS8 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTE1062
Plasmid#172356PurposeDetection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.DepositorInsertsUseSynthetic Biology; Integrative into streptomyces …TagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo VPS51-13Myc
Plasmid#175094Purpose13Myc tagged VPS51 is expressed in mammalian cellsDepositorInsertVPS51 (VPS51 Human)
UseTags13MycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLF063: Lenti-fCAG-jRGECO1a
Plasmid#124775PurposeFor general lentiviral or direct expression of cytosolic jRGECO1aDepositorInsertjRGECO1a
UseLentiviralTagsNES-6xHISExpressionMammalianMutationPromoterCAGAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP443
Plasmid#216361PurposexNup98-Nb1t (+1Cys) Nanobody productionDepositorInsertxNup98-Nb1t (+1Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
UseTagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBintN
Plasmid#48135PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo VPS54-13Myc
Plasmid#175095Purpose13Myc tagged VPS54 is expressed in mammalian cellsDepositorInsertVPS54 (VPS54 Human)
UseTags13MycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Bax1-2/L-6
Plasmid#30533DepositorInsertBCL2-associated X protein (BAX Human)
UseTagseGFPExpressionMammalianMutationF30C E44C C62S L63C C126S P130CPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
EGFP-mCherry-Ki67
Plasmid#183750PurposeExpresses EGFP-mCherry-Ki67DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
hOC-GFPtpz
Plasmid#110207PurposeExpresses GFP in mature bone cells.DepositorInserteGFPtopaz
UseTagsNoneExpressionMammalianMutationPromoterHuman osteocalcin (BGLAP)Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Htb2-GFP
Plasmid#117669PurposeColorize the nucleiDepositorInsertHtb2-GFP
UseTagsExpressionYeastMutationContains the gene encoding the Histone Htb2 from …PromoterpGAPAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRU5HT1-inLuc
Plasmid#58954PurposeHTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.DepositorInsertinLuc
UseRetroviralTagsExpressionMutationPromoterCMV/R/U5Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only
