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Plasmid#180202PurposeExpresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cellsDepositorInsertAs-NF-kB3
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 2-412PromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Co-NF-kB-Cterm
Plasmid#180199PurposeExpresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cellsDepositorInsertCo-NF-kBCterm
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 542-1224PromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
iDianiSnFR
Plasmid#177741PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiDianiSnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a His6 GST PYL2(14-188)
Plasmid#177842PurposeBacterial Expression of PYL2DepositorInsertPYR1-like 2 (PYL2 Mustard Weed)
UseTags6xHis-GSTExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCytSnFR
Plasmid#177738PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCytSnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_BrEt_SnFR
Plasmid#177740PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_BrEt_SnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_F_SnFR
Plasmid#177739PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_F_SnFR
UseTagsHA, His, and MycExpressionBacterialMutationPromoterT7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO FAPP2-GLTPH
Plasmid#170739PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertPLEKHA8-GLTPH (PLEKHA8 Human)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-CreERT2 sgAdam10 v3
Plasmid#158047Purposelenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9DepositorInsertAdam10 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3a-(CC93)-ER
Plasmid#125121PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)-ER
UseTagsER retention motifExpressionMammalianMutationPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3b-(V7)-ER
Plasmid#125123PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(V7)-ER
UseTagsFusion protein -Endoplasmic retention motifExpressionMammalianMutationPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHHM.X513-iNicSnFR3a-(CC93)
Plasmid#124880PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)
UseTagsHA Tag and His TagExpressionBacterialMutationNAPromoterT7Available sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHHM.X513-iNicSnFR2-(CC90)
Plasmid#124836PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR2-(CC90)
UseTagsHA tags and His TagExpressionBacterialMutationNAPromoterT7Available sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2 neg ctrl
Plasmid#112030PurposeMode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)DepositorInsertNEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)
Plasmid#112802PurposeExpression of OCTN2 mutantDepositorInsertOCTN2/SLC22A5 (Slc22a5 Rat)
UseTags3 x FlagExpressionMammalianMutationamino acids 14_22 and 447_454 deletedPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS1207
Plasmid#29104PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle235
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
LAMP-mChF-AIP
Plasmid#61524PurposeExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIPDepositorInsertlysosomal-associated membrane protein 1 (LAMP1 Human)
UseTagsAIP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX NPC1-FLAG-TiD
Plasmid#199748PurposeLentiviral construct to stably express NPC1 TurboIDDepositorInsertNPC1 (NPC1 Human)
UseTagsFlag and TurboIDExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-cGASsh4
Plasmid#127646PurposeKnock-down of human cGASDepositorInsertcGAS shRNA (CGAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only