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Addgene

p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)
(Plasmid #112802)

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Item Catalog # Description Quantity Price (USD)
Plasmid 112802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p3 FLAG_CMV14
  • Backbone manufacturer
    Sigma
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OCTN2/SLC22A5
  • Alt name
    Organic cation/carnitine transporter
  • Species
    R. norvegicus (rat)
  • Mutation
    amino acids 14_22 and 447_454 deleted
  • Entrez Gene
    Slc22a5 (a.k.a. CT1, OCTN2, UST2r)
  • Tag / Fusion Protein
    • 3 x Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454) was a gift from Katarzyna Nalęcz (Addgene plasmid # 112802 ; http://n2t.net/addgene:112802 ; RRID:Addgene_112802)

  • For your References section:

    Caveolin-1--a novel interacting partner of organic cation/carnitine transporter (Octn2): effect of protein kinase C on this interaction in rat astrocytes. Czeredys M, Samluk L, Michalec K, Tulodziecka K, Skowronek K, Nalecz KA. PLoS One. 2013 Dec 13;8(12):e82105. doi: 10.1371/journal.pone.0082105. eCollection 2013. PONE-D-13-35323 [pii] PubMed 24349196
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