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We narrowed to 13,516 results for: pET

Showing: 1861 - 1880 of 13516 results

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986H

Plasmid

#174801

Purpose

Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986H (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986S

Plasmid

#174802

Purpose

Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986S (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G972R

Plasmid

#174800

Purpose

Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G972R (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

G972R, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET3d::strep-AID

Plasmid

#15924

Depositor

Nina Papavasiliou

Article

Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

Insert

AID (AICDA Human)

Use

Tags

strep

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pET23a-His-hRanGAP1tail

Plasmid

#53139

Purpose

E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanGAP1 (RANGAP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

C-terminal fragment (amino acids 398-587)

Promoter

T7

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pETSXM2-pLacI-mtrC

Plasmid

#174615

Purpose

Bacterial expression of mtrC from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

MtrC

Use

Synthetic Biology; Shuttle vector in e. coli and …

Tags

Expression

Bacterial

Mutation

Promoter

pLacI

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6_rpoA-C

Plasmid

#190966

Purpose

Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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wt 6XHis-SNX9 pET15b

Plasmid

#34690

Depositor

Sandra Schmid

Article

Yarar et al Traffic. 2008 Jan;9(1):133-46. Epub 2007

Insert

Sorting nexin 9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pET-NCBD + ACTR

Plasmid

#99335

Purpose

Bacterial coexpression of CBP NCBD and NCoA3 binding domains

Depositor

Peter Wright

Article

Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

Inserts

CBP NCBD (2059-2117) (CREBBP Mouse)
SRC3 (1023-1093) (NCOA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b LFn-Fla

Plasmid

#84871

Purpose

Bacterial expression of LFn-Fla fusion

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only
pET28_mouse-pro-IL18

Plasmid

#183389

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL18 (Il18 Mouse)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET41-GST-KaiC

Plasmid

#68051

Purpose

Strain: AM5117, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiC

Use

Tags

6xHis, GST, and S-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET-2HMG-eGFP

Plasmid

#112737

Purpose

Express 2HMG-eGFP in E. coli

Depositor

Kyubong Jo

Article

Lee et al RSC Adv, 2016, 6, 46291-46298

Insert

2HMG-eGFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-Ets1-ETS

Plasmid

#85735

Purpose

Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440

Depositor

Gregory Poon

Article

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Mouse, Human)

Use

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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Brg1 Del2 pET28a

Plasmid

#122116

Purpose

Expression Brg1 amino acids 1-837 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Use

Tags

6XHis and His-T7

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-YTHDF2

Plasmid

#204406

Purpose

E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET-ChE1 v4.5

Plasmid

#185755

Purpose

Bacterial expression of ChE1 v4.5

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

ChE1 v4.5

Use

Tags

His10

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET11a-p65(19-325)

Plasmid

#44744

Depositor

Gourisankar Ghosh

Insert

p65 (Rela Mouse)

Use

Tags

Expression

Bacterial

Mutation

AA 19-325

Promoter

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only