pETa(+) HpCsd3 (42-403)
(Plasmid
#71285)
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PurposeExpresses Csd3 from Helicobacter pylori
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21a(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 6529
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTo produce protein, transform into Rosetta2(DE3)pLysS cells.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHP0506
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Insert Size (bp)1086
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Entrez GeneHP0506 (a.k.a. HP0506)
- Promoter T7
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Tag
/ Fusion Protein
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETa(+) HpCsd3 (42-403) was a gift from SeWon Suh (Addgene plasmid # 71285 ; http://n2t.net/addgene:71285 ; RRID:Addgene_71285) -
For your References section:
Structure of Csd3 from Helicobacter pylori, a cell shape-determining metallopeptidase. An DR, Kim HS, Kim J, Im HN, Yoon HJ, Yoon JY, Jang JY, Hesek D, Lee M, Mobashery S, Kim SJ, Lee BI, Suh SW. Acta Crystallogr D Biol Crystallogr. 2015 Mar;71(Pt 3):675-86. doi: 10.1107/S1399004715000152. Epub 2015 Feb 26. 10.1107/S1399004715000152 PubMed 25760614