-
Plasmid#155151PurposeExpresses Strep-MlaF-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationadded Streptavidin tag on MlaF N-terminusPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2074
Plasmid#155155PurposeExpresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaB_T52EPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2076
Plasmid#155156PurposeExpresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_K47APromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2077
Plasmid#155157PurposeExpresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_E170QPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2078
Plasmid#155158PurposeExpresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_Y261APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2079
Plasmid#155159PurposeExpresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_L265APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1244
Plasmid#155138PurposeExpresses MlaFED operon in E. coliDepositorInsertmlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Yes-10kb
Plasmid#112996PurposeP element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genomeDepositorInsert10Kb DAFC-62D amplicon
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSABAD518
Plasmid#46374DepositorInsertTvoVMA intein, inactive
UseTagsH6-GB1 and SH3ExpressionBacterialMutationN186A, T+1A mutations, inactivePromoterpBADAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG-deltaA
Plasmid#33151DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
UseTagsLacZExpressionYeastMutationThe RP51A sequence was modified by digestion with…PromoterAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a207-368.wt
Plasmid#13531DepositorInsertB. subtilis DEAD-box helicase YxiN second domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 1-206 and 369-479PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2
Plasmid#221225PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporterDepositorInsertAID-PcrA M6-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
ERM-APEX2
Plasmid#79055PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.DepositorInsertAPEX2 anchored to the cytosolic face of the ER membrane
UseLentiviralTagsResidues 1-27 of P450 oxidase 2C1 and V5ExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE1062
Plasmid#172356PurposeDetection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.DepositorInsertsUseSynthetic Biology; Integrative into streptomyces …TagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-BLM-UGI-T2A-mTagBFP2
Plasmid#221222PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporterDepositorInsertAID-BLM-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-tadDE-PcrA M6-UGI
Plasmid#221236PurposeExpress HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.DepositorInserttadDE-PcrA M6-UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115268PurposeExpresses EiCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertEiCsm6
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_XS3B-miniXon_Luciferase
Plasmid#217302PurposePlasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by LDepositorInsertXS3B-miniXon
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only