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Showing: 3721 - 3760 of 149038 results

  3. AICSDP-26:ST6GAL1-mEGFP

    Plasmid
    #101786
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1
    Depositor
    Allen Institute for Cell Science
    Insert
    ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101786-plasmid-map-sequence-id-197675

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  5. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  11. pNO286-3

    Plasmid
    #107746
    Purpose
    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)
    Depositor
    Jeffrey Tabor
    Article
    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb
    Inserts
    CcaS
    Ho1
    PcyA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Deleted AA 223 and 512
    Promoter
    J23106 and J23108
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107746-plasmid-map-sequence-id-209202

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  14. qTAG-C-V5-Zeo

    Plasmid
    #207741
    Purpose
    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207741-plasmid-map-sequence-id-413810

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  26. TFORF0161

    Plasmid
    #142546
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MLLT1 (MLLT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142546-plasmid-map-sequence-id-395949

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  29. pGEX-2T-Rac1-wt

    Plasmid
    #12977
    Depositor
    Gary Bokoch
    Insert
    Rac1 (RAC1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  31. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid
    #49077
    Purpose
    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x HA and mCerulean
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49077-plasmid-map-sequence-id-72205

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  35. pAAV-Syn-SomaGCaMP6f2

    Plasmid
    #158756
    Purpose
    Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158756-plasmid-map-sequence-id-310660

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Showing: 3721 - 3760 of 149038 results