Addgene

We narrowed to 47,817 results for: ANG

Showing: 2791 - 2820 of 47817 results

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pYL-kasOp-FnCas12a3

Plasmid

#177875

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized the FnCas12a mutant EP16

Use

CRISPR

Tags

Expression

Mutation

Promoter

kasOp*

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKI-GFP

Plasmid

#118482

Purpose

GFP-tagged Protein Kinase A inhibitor peptide.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

PKI-GFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-blueAKAR

Plasmid

#118470

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP

Plasmid

#99731

Purpose

overexpression of PDGFRa and CopGFP

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Pdgfra (Pdgfra Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP63_sgRNA-1

Plasmid

#88854

Purpose

CRISPR KO of Trp63

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp63

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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UPB-duodenum (UPB6)

Plasmid

#180785

Purpose

Unique Projection Barcode (UPB6)-Duodenum for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM4D(Gi) (partial )

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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S3_001_026_MV2

Plasmid

#134422

Purpose

pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Use

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-FRISZ

Plasmid

#176887

Purpose

Far-red fluorescent zinc indicator FRISZ in pDisplay

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Use

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Mito-AcGFP-MTn

Plasmid

#154350

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ost4-GFP-MTn

Plasmid

#154351

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL638

Plasmid

#189831

Purpose

The T-DNA backbone of nCas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

Expression

Mutation

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSBY3_ligD-HygR

Plasmid

#104625

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

FnCpf1 codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR

Plasmid

#118464

Purpose

cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-MTn-KDEL

Plasmid

#154348

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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PBDGTV

Plasmid

#100859

Purpose

A piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.

Depositor

Yue Huang

Article

Liu et al Nucleic Acids Res. 2017 Sep 28. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC MYC hTel2

Plasmid

#22802

Depositor

Titia de Lange

Article

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (TELO2 Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pGL3-U6-sgRNA_SRD5A3-mcherry

Plasmid

#159782

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A3 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting SRD5A3 gene (SRD5A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-peg

Plasmid

#141081

Purpose

Gateway compatible prime editing gRNA empty backbone vector

Depositor

Yiping Qi

Article

Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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UPB-pancreas (UPB7)

Plasmid

#180787

Purpose

Unique Projection Barcode (UPB7)-Pancreas for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM4D(Gi) (partial )

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-2AFP-KDEL

Plasmid

#154349

Purpose

Mammalian expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pNEXdelta-EGFP-ApAF

Plasmid

#13335

Depositor

Bong-Kiun Kaang

Article

Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

Insert

Aplysia activating factor

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
pNEXdelta-ApAF

Plasmid

#13334

Depositor

Bong-Kiun Kaang

Article

Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

Insert

Aplysia activating factor

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
Lyn-FRB-GFP11

Plasmid

#181863

Purpose

Plasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

Lyn-FRB-GFP11

Use

Tags

GFP11 and N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJIT163-eAFID-3

Plasmid

#167360

Purpose

For inducing predictable deletions in protoplasts and wheat plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3Bctd-Cas9-UDG-T2A-AP lyase

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCA24-pHR-CMV-stdMCP-p65-HSF1-T2A-GFP

Plasmid

#199455

Purpose

Expression of stdMCP-PH in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdMCP-PH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pH-AFID-3

Plasmid

#167361

Purpose

For inducing predictable deletions in rice plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3A-Cas9-UDG-T2A-AP lyase

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Stephen Lory, Cammie Lesser

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP

Plasmid

#199456

Purpose

Expression of stdPCP-PH in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdPCP-PH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorPE-FRISZ

Plasmid

#176888

Purpose

Far-red fluorescent zinc indicator FRISZ in pTorPE vector

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Use

Tags

Periplasmic expression TorA tag and Twin-Strep-ta…

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_SRD5A1-mcherry

Plasmid

#159780

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge