-
Plasmid#61838PurposeExpresses GST-tagged anti-GFP Nanobody in E. coli cells.DepositorInsertGFP-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-eGFP-C1-VPS35
Plasmid#163622Purposehuman VPS35 cDNA C-terminally tagged with eGFPDepositorInserthuman VPS35 (VPS35 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MEKK1 WT
Plasmid#12181DepositorInsertMEKK1 (Map3k1 Mouse)
UseTagsHis and XpressExpressionMammalianMutationV1467LPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR
Plasmid#191795Purposevector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6DepositorInserthumanized hfCas13d
UseAAV and CRISPRTagsExpressionMammalianMutationA134V, A140V, A141V, A143VPromoterEFS, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
1098 pcDNA GFP FKHR
Plasmid#9022DepositorInsertFKHR (FOXO1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV
Plasmid#72265PurposeExpress gene of interest under CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57.1-MAT2A
Plasmid#100521PurposeDox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)DepositorInsertMAT2A (MAT2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpTight TREAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ef1a_Large T-antigen_Ires_Puro
Plasmid#18922DepositorInsertLarge T Antigen (SV40gp6 Simian virus 40)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FOXO3a TM
Plasmid#8361DepositorInsertFOXO3a (FOXO3 Human)
UseTagsFlagExpressionMammalianMutationT32A, S253A, S315APromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MEKK1 KD
Plasmid#12180DepositorInsertMEKK1 (Map3k1 Mouse)
UseTagsHis and XpressExpressionMammalianMutationKinase dead: K1253M; V1467LPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
Plasmid#188777PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag FoxO3
Plasmid#14937DepositorInsertFoxO3 (FOXO3 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GP
Plasmid#60511PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1363 LV EF1a-CD19 IRES-EGFP
Plasmid#201919Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD19DepositorInsertCD19 (CD19 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3550
Plasmid#145026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tet3G blasticidin
Plasmid#128061PurposeLentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCXWB-EBNA1
Plasmid#37624PurposeNon-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmidsDepositorInsertEBNA-1 (EBNA-1 human herpesvirus 4)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only