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Plasmid#225719PurposeTransfection of USP27X (G76S) with HA tag in mammalian cellsDepositorInsertUSP27X G76S (USP27X Human)
UseTagsHAExpressionMammalianMutationG76SPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro hGSDME gRNA2
Plasmid#223520PurposeKnocking out hGSDME in human cellsDepositorInsertGSDME (GSDME Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro hGSDME gRNA1
Plasmid#223519Purposeknocking out hGSDME in human cellsDepositorInsertGSDME (GSDME Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-12S-E1A-HA-puro
Plasmid#212671PurposeExpress adenovirus E1ADepositorInsert12S-E1A
UseLentiviralTagsHAExpressionMutationPromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR2_DAZL-T2A-mGreenLantern-PuroTK
Plasmid#222917PurposeHomology directed repair template for knocking in mGreenLantern reporter to DAZL.DepositorInsertmGreenLantern
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-PuroR
Plasmid#196962PurposeExpresses PE2 with puromycin resistanceDepositorInsertPE2-P2A-Puro
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR_NANOS3-T2A-mVenus-PuroTK
Plasmid#222903PurposeHomology directed repair template for knocking in mVenus reporter to NANOS3.DepositorInsertmVenus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR_NPM2-T2A-mGreenLantern-PuroTK
Plasmid#222910PurposeHomology directed repair template for knocking in mGreenLantern reporter to NPM2.DepositorInsertmGreenLantern
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR_FIGLA-T2A-mGreenLantern-PuroTK
Plasmid#222905PurposeHomology directed repair template for knocking in mGreenLantern reporter to FIGLA.DepositorInsertmGreenLantern
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro XLone-CCND2
Plasmid#179845Purposedonor plasmid for inducible expression of CCND2 in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR, Luciferase, and TALEN ; Donor plasmidTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Neuroligin-2 [L107/95R]
Plasmid#188184PurposeMammalian Expression Plasmid of anti-Neuroligin-2 (Mouse). Derived from hybridoma L107/95DepositorInsertanti-Neuroligin-2 (Mus musculus) recombinant mouse monoclonal antibody (Nlgn2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-BbsI-GGsite-mCherry-Puro
Plasmid#221232PurposeCloning vector for sgRNA with mCherry and PuroDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE 3xHA-MKK6-EE puro
Plasmid#216791PurposeMammalian expression of constitutively active human MKK6DepositorInsertMKK6 (MAP2K6 Human)
UseRetroviralTags3x-HAExpressionMammalianMutationS207E and T211E, constitutively activePromoterLTRAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-TET2Puro-gal4DBD-miniVPR-HA
Plasmid#207169PurposeDox Inducible Expression Plasmid for gal4DBD-miniVPR-HADepositorInsertGal4DBD-miniVPR
UseLentiviral and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-tet2puro-gal4DBD-HIFCAD
Plasmid#207173PurposeDox Inducible Expression Plasmid for gal4DBD-HIF-CADDepositorInsertGal4DBD-HIF1a-CAD
UseLentiviral; Dox inducibleTags2x MycExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP-2A-Puro-gATRIP_A
Plasmid#211537PurposesgRNA-A against ATRIPDepositorInsertsgRNA ATRIP
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-P2A-puro
Plasmid#214358PurposeExpresses SpCas9 and puromycin resistance genesDepositorInsertSpCas9 and puromycin resistance genes
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p21(RxL)-mCerulean-PuroR
Plasmid#215078PurposeExpresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.DepositorInsertCDKN1A (CDKN1A Human)
UseRetroviralTagsmCeruleanExpressionMammalianMutationp21(RxL) denotes mutations: R19A, R20A, L21A, F22APromoterLTRAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p21(dPCNA)-mCerulean-PuroR
Plasmid#215079PurposeExpresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.DepositorInsertCDKN1A (CDKN1A Human)
UseRetroviralTagsmCeruleanExpressionMammalianMutationp21(dPCNA) denotes mutations M147A, D149A, F150A.PromoterLTRAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only