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Plasmid#172143PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*); [strong promoter eG, strong RBS SD8] …PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH190
Plasmid#172148PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*); [strong promoter eG, strong RBS SD8] …PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-sgHPDL #3
Plasmid#174165Purposeknock out HPDL in human cell linesDepositorInsertsgRNA against HPDL
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-sgHPDL #9
Plasmid#174166Purposeknock out HPDL in human cell linesDepositorInsertsgRNA against HPDL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-Tev
Plasmid#84555PurposeMESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev proteaseDepositorInsertRap-MESA-FKBP-20-Tev
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher
Plasmid#157674PurposeExpression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertSpyCatcher with ddFLN4, ELP, Histag and ybbR tag
UseTagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-His-SpyTag-XMod(XL2V)-Doc
Plasmid#157676PurposeExpression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.DepositorInsertXMod-Doc mutant XL2V with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165V
Plasmid#158965PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165V
UseTagsExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165R
Plasmid#158966PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165R
UseTagsExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG
Plasmid#139963PurposeOver-expression of native 3-hydroxy-3-methyl glutaryl CoA reductaseDepositorInsertConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_2pabPAC(Y72L)
Plasmid#158970PurposeA negative control for AAV-mediated 2P-activatable bPACDepositorInsert2pabPACY72L
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM54
Plasmid#154157PurposeFirefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB siteDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoter8 copies of the Aedes aegypti Rel2 NF-κB siteAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM44
Plasmid#154153PurposeFirefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoterDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterDrosophila melanogaster minimal Hsp70 promoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
P302-FBP_1
Plasmid#139692PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P313-FBP_8
Plasmid#139699PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3HDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-vdh
Plasmid#128130PurposeExpressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-HipO
Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MRLC-DD-pIRES-EGFP-AP4-mito
Plasmid#101287PurposeTo define the role of Mena/VASP in tension-sensitive actin assemblyDepositorInsertMRLC-DD and AP4-Mito
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR1323
Plasmid#111125PurposeExpresses non-targeting_01224 sgRNA in pCR1068DepositorInsertNon-targeting gRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only