Addgene

We narrowed to 14,726 results for: grna

Showing: 1841 - 1860 of 14726 results

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pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

Plasmid

#182151

Purpose

To insert attB-rev attachment site at IDS in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS-C2c-attBrev pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd

Plasmid

#182150

Purpose

To install attP-fwd attachment site at IDS2 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS2-B7b-attP-fwd pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFREE2-eSpCas9-sgRNA

Plasmid

#179580

Purpose

Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

eSpCas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHu6-gRNA-NT1

Plasmid

#81203

Purpose

hU6 expression of gRNA NT1

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA NT1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3048(gRNA XII-2)

Plasmid

#73290

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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VKG1-gRNA-pX330

Plasmid

#108671

Purpose

Cleavage of VKG1 sequence by CRISPR/Cas9

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

gRNA for VKG1 sequence

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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SGK3 gRNA (BRDN0001146019)

Plasmid

#75540

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C8orf44-SGK3,SGK3 (SGK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SGK3 gRNA (BRDN0001146281)

Plasmid

#75541

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C8orf44-SGK3,SGK3 (SGK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB2 gRNA (BRDN0001147469)

Plasmid

#77546

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB2 (EPHB2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB2 gRNA (BRDN0001147792)

Plasmid

#77547

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB2 (EPHB2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB2 gRNA (BRDN0001147198)

Plasmid

#77548

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB2 (EPHB2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB4 gRNA (BRDN0001144879)

Plasmid

#76352

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB4 (EPHB4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EPHB4 gRNA (BRDN0001148436)

Plasmid

#76353

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB4 (EPHB4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LIN52 D5.4 gRNA

Plasmid

#90734

Purpose

3rd generation lentiviral gRNA plasmid targeting human LIN52

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

LIN52 (Guide Designation D5.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

Plasmid

#107731

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

Plasmid

#107728

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR1 gRNA (BRDN0001146517)

Plasmid

#76069

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGFR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGFR1 (FGFR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK2A gRNA (BRDN0001149371)

Plasmid

#75753

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2A (CAMK2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BMX gRNA (BRDN0001146364)

Plasmid

#75653

Purpose

3rd generation lentiviral gRNA plasmid targeting human BMX

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BMX (BMX Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-Esrrb gRNA

Plasmid

#128841

Purpose

gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Esrrb gRNA (Esrrb Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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