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We narrowed to 4,918 results for: pcDNA3.1+

Showing: 1821 - 1840 of 4918 results

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pcDNA3.1-MS2-HA-HshnRNPE1-del163-278_Z

Plasmid

#148117

Purpose

Mammalian Expression of HshnRNPE1-del163-278

Depositor

Elisa Izaurralde

Insert

HshnRNPE1-del163-278 (PCBP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPK-del210-387_Z

Plasmid

#148125

Purpose

Mammalian Expression of HshnRNPK-del210-387

Depositor

Elisa Izaurralde

Insert

HshnRNPK-del210-387 (HNRNPK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPM-del243-615_Z

Plasmid

#148128

Purpose

Mammalian Expression of HshnRNPM-del243-615

Depositor

Elisa Izaurralde

Insert

HshnRNPM-del243-615 (HNRNPM Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutations compared to the sequence of …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nmyc-Eps8L2_1-496-siR

Plasmid

#196515

Purpose

Expression of Myc-Eps8L2_1-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-496 (EPS8L2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG

Plasmid

#185347

Purpose

Membrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tag

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Cue1p/Ube2G2 fusion (internal MYC tag)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-myc-OGA(GS-544)C181_NES

Plasmid

#193998

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181_NES

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181_NES

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1 with cleavable His tag

Plasmid

#181813

Purpose

Expresses human MUC2 D1 with cleavable His tag, residues 21-389

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc2 D1 (MUC2 Human)

Use

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-p15 Bid-KKAA-GFP

Plasmid

#191970

Purpose

Expression of the active p15 cBid KKAA mutant, chimera with GFP.

Depositor

Luca Scorrano

Insert

p15 - cBid KKAA

Use

Tags

Expression

Mammalian

Mutation

KKAA: Lys 157 and Lys 158 into Ala

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

Plasmid

#181854

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Histidine 33 in DnaJB1 changed to glutamine.

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

Plasmid

#172583

Purpose

For transient expression of C-terminal BirA-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Tags

BirA(R118G)-HA

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

Plasmid

#172582

Purpose

For transient expression of C-terminal BirA-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Tags

BirA(R118G)-HA

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-del4-20_X

Plasmid

#147945

Purpose

Mammalian Expression of HsNanos3-del4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-del4-20 (NANOS3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

Plasmid

#148420

Purpose

Mammalian Expression of HsRck_296-472-10xmut

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-10xmut (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-kappa-myc-dK-2xG4S-TMst

Plasmid

#145766

Purpose

Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.

Depositor

Marcel Bruchez

Article

Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021

Insert

kappa-myc-dK-2xG4S-TMst

Use

Tags

Expression

Mammalian

Mutation

The dK FAP is dL5 with mutations at E50K, L89S, a…

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG F405G

Plasmid

#169956

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

F405G

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287S)

Plasmid

#172644

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287S) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ser

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286I)

Plasmid

#172645

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286I) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ile

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)

Plasmid

#172646

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Leu

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG R394E

Plasmid

#169954

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (R394E)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R394E

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

Plasmid

#169958

Purpose

Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG and Ub

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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Showing: 1821 - 1840 of 4918 results

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