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Plasmid#116731PurposeLentiviral expression of EGFRDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b RNase A
Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
UseTagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
GLASTp-DsRed2
Plasmid#17706DepositorInsertGLASTp (SLC1A3 Human)
UseTagsDsRed2ExpressionMammalianMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1
Plasmid#138461Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64DepositorInsertMCP-VP64-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL-pRc/CMV
Plasmid#19999DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationwild typePromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV LIC cloning vector (1B)
Plasmid#29653DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)
Plasmid#179115PurposeCargo EGFP with AttP Bxb1 site (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHSE401
Plasmid#62201PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-R5-GFP
Plasmid#17838DepositorInsertpET30-GFP
UseSynthetic BiologyTagsHis, R5, and S-tagExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-HIF1B (#930)
Plasmid#99916Purposemammalian expressionDepositorInsertHIF1B (ARNT Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCE-hOCT3/4
Plasmid#41813PurposeNon-integrating (episomal) expression of human OCT3/4DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA H1047R
Plasmid#12524PurposeRetroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationH1047R. Tumor-derived allele.PromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-csGBP
Plasmid#108875PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)DepositorInsertmKate2-P2A-APEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_erGAP3
Plasmid#78118PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorInserterGAP3
UseTagsKDEL and calreticulin signal peptideExpressionMammalianMutationS175G, D180Y, I167V and L15Q in GFP moiety and D…PromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGM3B
Plasmid#110809Purposecontains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genesDepositorInsertAAV3B cap genes, AAV2 rep genes, adenovirus helper genes
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-V5-TurboID(N)-GPI
Plasmid#163696PurposeExpresses n-terminal half of TurboID on surface of neuronsDepositorInsertGPI anchored TurboID
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Str-Ii_SBP-mCherry-Golgin84
Plasmid#65304Purposesynchronize trafficking of Golgin-84 from the ER (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only