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Plasmid#160073PurposeZ. mobilis CRISPRi, promoter A, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pJK398
Plasmid#155370PurposedCas9 sgRNA oscillator v1 (dCRv1) (p15A)DepositorInsertdCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-3XBFPnls
Plasmid#75384PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianMutationPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTarget12f1
Plasmid#171183PurposeConstitutive expression of sgRNA without donor DNADepositorInsertsgRNA scaffold in the CRISPR/Cas12f1 system
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119(SpeI) promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-3XNeon-nls
Plasmid#75386PurposePCP-3XNeon-nlsDepositorInsertPCP
UseLentiviralTags3XmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-GMR-eya(shRNA)
Plasmid#157991PurposepBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP618
Plasmid#169235PurposeSpear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adaptersDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDCC6
Plasmid#59985PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassetteDepositorInsertsCas9
U6-2>sgRNA
UseCRISPRTags3xFLAGExpressionMutationPromoterU6-96Ab and hsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-N22p-3XRFPnls
Plasmid#75387PurposeN22p-3XRFPnlsDepositorInsertN22p
UseLentiviralTags3XmCherryExpressionMammalianMutationPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgAAVS1
Plasmid#184403PurposepX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGATDepositorInsertAAVS1-specific sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRGE31
Plasmid#50929PurposeExpressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRice snoRNA U3 and dual 35S promoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAK008
Plasmid#128225Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 2DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 2
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK009
Plasmid#128226Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 3DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 3
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIKU801-CrPEPC1
Plasmid#92342PurposeExpresses sgRNA for targeting CrPEPC1 gene, paromomycin resistanceDepositorInsertsgRNA-CrPEPC1
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-MCP-3XBFPnls
Plasmid#75383PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKHH030
Plasmid#89358PurposehU6 sgRNA cloning vector for CDKO systemDepositorInsertGFP, Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA-AAVS1_hspCas9
Plasmid#193309PurposeCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-3XGFPnls
Plasmid#75385PurposePCP-3XGFPnlsDepositorInsertPCP
UseLentiviralTags3XsfGFPExpressionMammalianMutationPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_0
Plasmid#127553PurposeDrosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 0
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_1
Plasmid#127554PurposeDrosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 1
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only